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Showing: 641 - 660 of 28679 results

  1. pLEX307_CR-PDHA2S293D

    Plasmid
    #115201
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S293D (PDHA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    July 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    115201-plasmid-map-sequence-id-231792

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  2. pCJH026_proD_Cas12c-multiplexing_CAM

    Plasmid
    #183073
    Purpose
    Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression
    Depositor
    Jennifer Doudna
    Article
    Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4
    Insert
    Expressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183073-plasmid-map-sequence-id-358157

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  3. pLeGO.sgGata1.4.RUNX1A.iG2

    Plasmid
    #181976
    Purpose
    gene knock out of mouse GATA1, overexpression of 3xFLAG-RUNX1A (human)
    Depositor
    Jan-Henning Klusmann
    Inserts
    GATA1 gRNA: CCTAGACCAGGAAAATCCAT (Gata1 Mouse)
    RUNX1A (RUNX1 Human)
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mutation
    Promoter
    Available since
    April 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181976-plasmid-map-sequence-id-358965

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  13. pLEX307_CR-PDHA2S293A

    Plasmid
    #115204
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S293A (PDHA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115204-plasmid-map-sequence-id-231794

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  15. pLEX307_CR-PDHA2

    Plasmid
    #115199
    Purpose
    Lentiviral transduction and expression of a CRISPR/Cas9-resistant PDHA2 into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2WT (PDHA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115199-plasmid-map-sequence-id-232425

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  16. pLEX307_CR-PDHA2S291A

    Plasmid
    #115203
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115203-plasmid-map-sequence-id-232429

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Showing: 641 - 660 of 28679 results