Skip to main content
Addgene
Showing: 641 - 660 of 5499 results

  1. pαH-S-GSAS-B.1.617.2.v1

    Plasmid
    #182575
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein Delta variant, furin side replaced by GSAS, 2 Proline at 986 and 987 plus T19R-G142D-del156/157-R158G-L452R-T478K-D614G-P681R-D950N
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike S-GSAS-B.1.617.2.v1 (T19R-G142D-Del156/157-R158G-L452R-T478K-D614G-P681R-D950N) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    182575-plasmid-map-sequence-id-353646

    Enlarge

  2. pαH-rS2d-HexaPro

    Plasmid
    #183515
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein rS2d-HexaPro with (682-685) furin side replaced with GSAS and mutation S383C, D985C, F817P, A892P, A899P, A942P, K986P,V987P
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-rS2d.HexaPro) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    May 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    183515-plasmid-map-sequence-id-362649

    Enlarge

  3. pGBW-m4134900

    Plasmid
    #151911
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilized
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    d1-15 d1209-1273 R682A R685G K986P V987P
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    151911-plasmid-map-sequence-id-300222

    Enlarge

  4. pGBW-m4134904

    Plasmid
    #151930
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 spike prefusion-stabilized
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 spike prefusion-stabilized (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    d1-15 d1209-1273 R682A R685G K986P V987P
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    151930-plasmid-map-sequence-id-297934

    Enlarge

  5. pGBW-m4258326

    Plasmid
    #154793
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 surface glycoprotein (Spike protein) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;V5
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    July 30, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154793-plasmid-map-sequence-id-311545

    Enlarge

  6. pαH-S-GSAS-2P-OMICRON

    Plasmid
    #180593
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180593-plasmid-map-sequence-id-352871

    Enlarge

  7. pαH-S-GSAS-OMICRON

    Plasmid
    #180423
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RR…
    Promoter
    Available since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    180423-plasmid-map-sequence-id-354264

    Enlarge

  9. Anti-GCN4 [IPI-C11L34] (Recombinant Antibody)

    Antibody
    #218104-rAb
    Purpose
    Anti-GCN4 tag chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds to HLENEVARLKK sequence.
    Depositor
    Institute for Protein Innovation (IPI)
    Article
    Zahnd et al J Biol Chem. 2004 Apr 30;279(18):18870-7
    Recommended Applications
    Western Blot
    Reactivity
    Budding yeast
    Source Species
    Rabbit
    Isotype
    IgG1
    Trial Size
    Not available to purchase
    Available since
    May 14, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    Western Blot image by Institute for Protein Innovation (IPI)

  11. AAVS1_puro_CAG_HexaPro

    Plasmid
    #164077
    Purpose
    Targeted integration of SARS-CoV-2 spike HexaPro variant into the AAVS1 genomic safe harbor locus
    Depositor
    Yannick Doyon
    Article
    Rivest et al protocols.io 2021
    Insert
    SARS-CoV-2 S HexaPro (S Severe acute respiratory syndrome coronavirus 2)
    Use
    CRISPR
    Tags
    2X Strep-Tag II, 8X His tag, and HRV 3C cleavage …
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CAG
    Available since
    Jan. 4, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    164077-plasmid-map-sequence-id-320680

    Enlarge

  13. pUC19-TSV (linearized in VP2)

    Plasmid
    #70032
    Purpose
    pUC19-TSV (linearized in VP2) contains a genome of the Trichodysplasia spinulosa-associated polyomaviruse (GenBank NC_014361) linearized and cloned in the VP2-coding region
    Depositor
    Mariet Feltkamp
    Article
    van der Meijden et al PLoS Pathog. 2010 Jul 29;6(7):e1001024.
    Insert
    TSV full genome, linearized in VP2 gene
    Use
    Cloning vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    70032-plasmid-map-sequence-id-129213

    Enlarge

  20. pαH-S-HRV3C

    Plasmid
    #164567
    Purpose
    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site (S-HRV3C variant)
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:
    Insert
    Spike - Furin site changed for HRV3C site
    Use
    Tags
    2X Strep-Tag II and 8X His tag
    Expression
    Mammalian
    Mutation
    682-685 (furin site) replaced with HRV3C site (LE…
    Promoter
    Available since
    Feb. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164567-plasmid-map-sequence-id-324831

    Enlarge

Showing: 641 - 660 of 5499 results