We narrowed to 41,611 results for: KAN

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pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#1

Plasmid

#172983

Purpose

CRISPRi for TAOK2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

sgTAOK2i (TAOK2 Human)

Use

CRISPR and Lentiviral

Tags

3xFLAG

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-TdTdelta5cys

Plasmid

#207465

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variant

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTdelta5cys (Dntt Mouse)

Tags

10xHis

Expression

Bacterial

Mutation

Cys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pXD70DAmt1-DadR/DadS-Pdadh-DadhABC(R506S, A2)

Plasmid

#191631

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), native promoter-dopamine dehydroxylase from dopamine-metabolizing E. lenta A2 strain (R506S mutation) with transcriptional regulators DadR/DadS

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

Dopamine dehydroxylase (R506S mutation, inactive form)

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.0RTcgr-Pct5.1-crRNA(AarI)-RT(Δcgr12)

Plasmid

#191651

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(nontargeting spacer) cgr1/cgr2-deleting repair template, used as negative control for pXD71Cas10.4RT4

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

Plasmid

#190154

Purpose

Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp3 (Vamp2 Rat)

Use

AAV

Tags

P2AT2A-EGFP-caax

Expression

Mammalian

Mutation

Truncation that includes only rat Vamp3 AA #1-81

Promoter

MBP

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1/V5-HisA-RvCAHS8-GS-AcGFP1

Plasmid

#192470

Purpose

Transient expression of RvCAHS8 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS8

Tags

AcGFP1

Expression

Insect

Promoter

Ac5 promoter

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1/V5-HisA-RvCAHS3-GS-AcGFP1

Plasmid

#192469

Purpose

Transient expression of RvCAHS3 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3

Tags

AcGFP1

Expression

Insect

Promoter

Ac5 promoter

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEThT-RvCAHS8

Plasmid

#192467

Purpose

Bacterial expression of RvCAHS8

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS8

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEThT-RvCAHS3

Plasmid

#192466

Purpose

Bacterial expression of RvCAHS3

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-MMETn-del-gRNA2

Plasmid

#185007

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

MMETn-del-gRNA2

Use

CRISPR

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-IAP-del-gRNA1

Plasmid

#185002

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

IAP-del-gRNA1 (Akp3 Mouse)

Use

CRISPR

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-MMERVK9C-del-gRNA1

Plasmid

#185004

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

MMERVK9C-del-gRNA1

Use

CRISPR

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-MMETn-del-gRNA1

Plasmid

#185006

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

MMETn-del-gRNA1

Use

CRISPR

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-MMERVK9C-del-gRNA2

Plasmid

#185005

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

MMERVK9C-del-gRNA2

Use

CRISPR

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pX458-IAP-del-gRNA2

Plasmid

#185003

Purpose

expression of gRNA and Cas9 from S. pyogenes with 2A-EGFP

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

IAP-del-gRNA2 (Akp3 Mouse)

Use

CRISPR

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pJM621 EF1α TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 in TUPV3

Plasmid

#161572

Purpose

Constitutive expression of TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

TC 3xFLAG-FRB-FGFR4-PRS(M)-(GS)-NLS-DsDed-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM588 EF1α 3xFLAG-NLS-VP64-intC-ZF1-DsDed-NLS-HA in TUPV2

Plasmid

#161564

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-intC-ZF1-DsDed-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-intC-ZF1-DsDed-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJM590 EF1α 3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA in TUPV2

Plasmid

#161566

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-ZF1-intC-ZF10-NLS-HA

Use

Synthetic Biology

Tags

3x-FLAG and HA

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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CBSH3+ shRNA-2

Plasmid

#160068

Purpose

Knock Down SH3+ Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Promoter

mouse U6 promoter

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3+ shRNA-1

Plasmid

#160066

Purpose

Knock Down SH3+ Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Rat)

Use

RNAi

Promoter

mouse U6 promoter

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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