We narrowed to 59,082 results for: {{{s}}}

1. pAG32-3HA-3FLAG

   Plasmid

   #154081

   Purpose

   For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

   Depositor

   Cornelia Kilchert

   Article

   Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

   Insert

   3xHA-3xFLAG-hygR
   

   Use

   Pcr-based tagging in yeast

   Tags

   3xHA-3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-ER-EmEGFP

   Plasmid

   #169000

   Purpose

   Express ER-EmEGFP in zebrafish

   Depositor

   Noboru Mizushima

   Article

   Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

   Insert

   ER-EGFP (encoding the signal sequence of human calreticulin mRNA and EGFP-KDEL) (CALR Human, Synthetic)
   

   Use

   Unspecified

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-U6-sgRNA_VEGFA-mcherry

   Plasmid

   #159790

   Purpose

   S. pyogenes sgRNA collocated with pegRNA targeting human VEGFA gene

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of sgRNA targeting VEGFA gene (VEGFA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-U6-sgRNA_HOXD13-mcherry

   Plasmid

   #159793

   Purpose

   S. pyogenes sgRNA collocated with pegRNA targeting mouse HOXD13 gene

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of sgRNA targeting HOXD13 gene (HOXD13 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. M26V DJ-1 pET15b

   Plasmid

   #60688

   Purpose

   Recombinant protein expression of Park7

   Depositor

   Mark Wilson

   Article

   Milkovic et al Protein Sci. 2015 Oct;24(10):1671-85. do

   Insert

   M26V DJ-1 (PARK7 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M26V

   Promoter

   T7

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pUDP010

   Plasmid

   #101166

   Purpose

   E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   HH-gRNA-HDV targetting SeILV6 in S. pastorianus
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-p53-G609T

   Plasmid

   #229531

   Purpose

   Expresses p53 mutant CASM203 in mamallian cells

   Depositor

   Robin Fåhraeus

   Article

   Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

   Insert

   p53-CDS (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.G609T

   Promoter

   CMV

   Available since

   July 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-p53-G609A

   Plasmid

   #229532

   Purpose

   Expresses p53 mutant G609A in mamallian cells

   Depositor

   Robin Fåhraeus

   Article

   Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

   Insert

   p53-CDS (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.G609A

   Promoter

   CMV

   Available since

   July 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-p53-G609C

   Plasmid

   #229533

   Purpose

   Expresses p53 mutant G609C in mamallian cells

   Depositor

   Robin Fåhraeus

   Article

   Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

   Insert

   p53-CDS (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   c.G609C

   Promoter

   CMV

   Available since

   July 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL3-U6-sgRNA_FANCF-mcherry

    Plasmid

    #159786

    Purpose

    S. pyogenes sgRNA collocated with pegRNA targeting human FANCF gene

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of sgRNA targeting FANCF gene (FANCF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. SP-dCas9-VPR

    Plasmid

    #63798

    Purpose

    SP-dCas9 with VP64-p65-Rta (VPR) fused to C-terminus; mammalian vector

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    SP-dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-G-Flamp1

    Plasmid

    #188567

    Purpose

    green biosensor for cAMP in mammalian cells

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-G-Flamp1-mut

    Plasmid

    #188568

    Purpose

    control probe of G-Flamp1, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1-mut
    

    Use

    Tags

    His tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti-SpCas9 neo

    Plasmid

    #104996

    Purpose

    This lentiviral construct delivers hSpCas9 and G418 resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lentiCRISPRv2 hygro

    Plasmid

    #98291

    Purpose

    Variant of lentiCRISPRv2 that confers hygromycin resistance

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLKO-Tet-On-shRNA-Control

    Plasmid

    #98398

    Purpose

    Lentivirus for expression of non-targeting control shRNA (Dox-inducible)

    Depositor

    Roland Friedel

    Article

    Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

    Insert

    non-targeting randomized sequence
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. LV-AR

    Plasmid

    #213819

    Purpose

    Lenti-viral expression of human androgen receptor wild-type (WT).

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR (human androgen receptor) (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag (N terminal on backbone)

    Expression

    Mammalian

    Mutation

    No, it is AR wild-type.

    Promoter

    EF-1a

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. ALDH18A1

    Plasmid

    #155402

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    ALDH18A1 (ALDH18A1 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS1099: Dual-sgRNA.Design 4

    Plasmid

    #159537

    Purpose

    Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

    Depositor

    Erik Sontheimer

    Article

    Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

    Insert

    Nme2Cas9 with two guide RNA cassettes
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lenti-SpCas9 hygro

    Plasmid

    #104995

    Purpose

    This lentiviral construct delivers hSpCas9 and hygromycin resistance.

    Depositor

    Brett Stringer

    Article

    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

    Insert

    KpnI-XhoI-BsrGI-NheI
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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