We narrowed to 10,563 results for: ESP

1. pCS2+8-CYP2A6(C82A, C439A)-flag-tev-halo-his

   Plasmid

   #227163

   Purpose

   For T-REX experiments of CYP2A6 C82A and C439A double mutant. Almost no sensing ability to reactive electrophilic species (RES).

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   CYP2A6 (CYP2A6 Human)
   

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Mutation

   changed cysteine 82 and cysteine 439 to alanine

   Promoter

   CMV and SP6

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2+8-PCSK1-flag-tev-halo-his

   Plasmid

   #227155

   Purpose

   For T-REX expriments of PCSK1.

   Depositor

   Yimon Aye

   Article

   Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

   Insert

   PCSK1 (PCSK1 Human)
   

   Tags

   Flag, HaloTag, and His6

   Expression

   Mammalian

   Promoter

   CMV and SP6

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. TOPO-LG4_chr5:550kb antisense

   Plasmid

   #205468

   Purpose

   Enhancer containing plasmid used for assessing enhancer::promoter interactions

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   chr5:550kb LG4
   

   Use

   Synthetic Biology; Subcloning of taq-amplified pc…

   Available Since

   July 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 2xFLAG-2xSTREP-KEAP1 (human)

   Plasmid

   #236422

   Purpose

   transient overexpression of KEAP1 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Marzio et al Cell. 2022 Jan 6;185(1):169-183.e19. doi

   Insert

   KEAP1 (KEAP1 Human)
   

   Expression

   Mammalian

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_ER-RE_MLPmin_BC1064-luc2

   Plasmid

   #227110

   Purpose

   Estrogen receptor response element for luciferase and barcode assays; barcode BC1064

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered ER response element linked to MLP minimal promoter driving barcode 1064 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-Grik1-GFP

   Plasmid

   #233645

   Purpose

   Expresses GFP in OFF cone bipolar cells

   Depositor

   Connie Cepko

   Article

   Avilés et al J Gen Physiol. 2025 Mar 3;157(2):e202413

   Insert

   Grik1 promoter (Grik1 Mouse)
   

   Use

   AAV

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF2479

   Plasmid

   #142947

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MESP2 (MESP2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_AR-RE_MLPmin_BC1013-luc2

   Plasmid

   #227107

   Purpose

   Androgen receptor response element for luciferase and barcode assays; barcode BC1013

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   8x clustered AR response element linked to MLP minimal promoter driving barcode 1013 and a luciferase reporter gene
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Jan. 31, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.23-WIPI1promotor

   Plasmid

   #206840

   Purpose

   The plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).

   Depositor

   Tassula Proikas-Cezanne

   Article

   Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

   Insert

   WIPI1 promotor (partial)
   

   Use

   Luciferase

   Available Since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR1A 2xHA GPR137

    Plasmid

    #226809

    Purpose

    Gateway entry vector for GPR137

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

    Insert

    GPR137 (GPR137 Human)
    

    Use

    Gateway entry

    Tags

    2xHA tag followed by a GDPPVAT linker

    Mutation

    Sequence optimized from NP_001164351

    Available Since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV_GR-RE_MLPmin_BC1093-luc2

    Plasmid

    #227113

    Purpose

    Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1093

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    12x clustered GR response element linked to MLP minimal promoter driving barcode 1093 and a luciferase reporter gene
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcs2-Δhel1/Δhel2HRI-GFP-IRES-mCherry

    Plasmid

    #226101

    Purpose

    HRI stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    HRI (EIF2AK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    SIFI degron helices 1 & 2 deleted (Δ61-112)

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcs2-N-HRI(1-138)-GFP-IRES-mCherry

    Plasmid

    #226100

    Purpose

    HRI N-terminal region (residues 1-138) stability reporter construct for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    HRI (EIF2AK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains only N-HRI (aa 1-138)

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-EF1A-Puro-mCherry-RAB7-S72A

    Plasmid

    #221556

    Purpose

    Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    Rab7A (RAB7A Human)
    

    Use

    Piggybac

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Change serine 72 to alanine

    Promoter

    EF1a

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

    Plasmid

    #221552

    Purpose

    Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human Rab7A (RAB7A Human)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIRHDGAL43C

    Plasmid

    #218279

    Purpose

    Introduction of a LowTempGAL Turbo module (HD-GAL43C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pNRG2>GAL4>tNRG2-PENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)-tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P), DHFR (P66L)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRHDGAL3C

    Plasmid

    #218276

    Purpose

    Introduction of a LowTempGAL Turbo module (HD-GAL3C) to accelerate induction of GAL promoter induction

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    fcy1(207-356)-pAgTEF1>ble>tAgTEF1-pENO2> UBI4>RHGSGTMV>DHFRP66L>G*6>GAL3C(S509P)>tENO2-fcy1(454-778)
    

    Expression

    Yeast

    Mutation

    GAL3 (S509P), DHFR (P66L)

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

    Plasmid

    #207136

    Purpose

    DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)
    

    Expression

    Mammalian

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. GFP Cav2.2 D122A pcDNA3

    Plasmid

    #206068

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEos3.2trLATm2allL+A

    Plasmid

    #203750

    Purpose

    Encodes the transmembrane domain from human LAT with mEos3.2 fused on the C terminus. Residues within the transmembrane domain mutated to L and A to increase the TMD interfacial surface area with the membrane. Used as a fluorescent plasma membrane probe.

    Depositor

    Sarah Veatch

    Article

    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

    Insert

    trLATm2(allL+A) (LAT Human)
    

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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