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pAAV-TNT4-Zfr-P2A-HA-YAP5SA

Plasmid

#223189

Purpose

Plasmid containing the Zfr/YAP5SA cassette. Translation of YAP5SA is controlled by splicing modulation of the Zfr by risdiplam.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Insert

YAP5SA (Yap1 Mouse)

Use

AAV

Tags

HA and engineered P2A

Mutation

S61A, S109A, S127A, S164A, S381A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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YjdC-R82A_pET-14b

Plasmid

#233276

Purpose

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

Depositor

Mohan Babu, Andrew Emili

Article

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

YjdC (yjdC )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 82…

Promoter

T7 promoter

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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YjdC-R64A_pET-14b

Plasmid

#233263

Purpose

Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

Depositor

Mohan Babu, Andrew Emili

Article

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

YjdC (yjdC )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 64…

Promoter

T7 promoter

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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HemH-R212A_pET-14b

Plasmid

#233262

Purpose

Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

Depositor

Mohan Babu, Andrew Emili

Article

Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

Insert

HemH (hemH )

Expression

Bacterial

Mutation

Arginine was replaced with Alanine at position 21…

Promoter

T7 promoter

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pML390_YWHAQ-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227925

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

YWHAQ homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML389_YWHAB-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227926

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

YWHAB homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML600_COG8-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227911

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

COG8 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML357_CAV1-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227914

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CAV1 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML361_DCP1A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227918

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

DCP1A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML366_HSPA1B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227920

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

HSPA1B homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML365_HSP90AA1-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227921

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

HSP90AA homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML364_PSMB7-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227922

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

PSMB7 homology arms with XTEN80-mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML374_CLTA-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227893

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CLTA homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML384_NCLN-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227897

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

NCLN homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML318_SEC23A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227903

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Article

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SEC23A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLJM1-NRBP1-FL-N-FLAG

Plasmid

#222675

Purpose

overexpressing full length NRBP1 with N-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

NRBP1 (NRBP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-TSC22D2-FL-N-FLAG

Plasmid

#222680

Purpose

overexpressing full length TSC22D2 with N-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM1-TSC22D2-FL-C-FLAG

Plasmid

#222681

Purpose

overexpressing full length TSC22D2 with C-terminal FLAG

Depositor

Jason Moffat

Article

Xiao et al Cell Rep. 2024 Jul 23;43(7):114417. doi:

Insert

TSC22D2 (TSC22D2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pVRC8400-SARS-CoV-2-RBD-BJ.1-AVI

Plasmid

#196617

Purpose

Expresses SARS-CoV-2 RBD protein from the BJ.1 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag

Depositor

Peter Kwong

Article

Teng et al PLoS One. 2022 May 24;17(5):e0268767. do

Insert

SARS-CoV-2-RBD-BJ.1

Tags

AVI, HRV3C, and scFc

Expression

Mammalian

Mutation

G339H, R346T, L368I, S371F, S373P, S375F, T376A, …

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pT2iApowriter2(#336)

Plasmid

#184104

Purpose

cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

Depositor

Sophie Vriz

Article

Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

Insert

myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2

Use

Zebrafish transient transgenesis

Tags

internal tag: 5myc

Mutation

DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

Promoter

Zf-Ubi

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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