We narrowed to 7,266 results for: 11

1. pLPCX mito Grx1-roGFP2

   Plasmid

   #64977

   Purpose

   Mammalian expression of mitochondrial Grx1-roGFP2 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   mitochondrial targeting sequence

   Promoter

   Available since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLPCX cyto Grx1-roGFP2

   Plasmid

   #64975

   Purpose

   Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

   Depositor

   Tobias Dick

   Article

   Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

   Insert

   Glutaredoxin-1 (GLRX Human)
   

   Use

   Retroviral

   Tags

   roGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. psiCHECK2-let-7 MT

   Plasmid

   #78261

   Purpose

   psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

   Depositor

   Joanne Weidhaas

   Article

   Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

   Insert

   mutated let-7 target site
   

   Use

   Luciferase; Microrna activity

   Tags

   Expression

   Mammalian

   Mutation

   nucleotides 2-5 and 10-11 changed from CTCC to GA…

   Promoter

   Available since

   June 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA

   Plasmid

   #164615

   Purpose

   Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.

   Depositor

   Jeffrey Chao

   Article

   Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.

   Insert

   Renilla luciferase
   

   Use

   Tags

   24xMS2v5 (3'UTR) and SunTag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   TetCMV

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRR.1

   Plasmid

   #69995

   Purpose

   Mammalian expression of cytochrome p450 CYP2B6

   Depositor

   Kathrin Klein

   Article

   Radloff et al Hum Mutat. 2013 May;34(5):725-34. doi: 1

   Insert

   CYP2B6 (CYP2B6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

   Plasmid

   #163024

   Purpose

   Expression of CRISPRi with U6-gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   AAV

   Tags

   KRAB, SV40 polyA, and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hEF-1a

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. SnapTag-Binder

   Plasmid

   #179142

   Purpose

   Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   SspB
   

   Use

   Tags

   SNAP-tag

   Expression

   Mammalian

   Mutation

   Y11K/A15E, residue numbering from pdb: 1ou9

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-mCherry-Binder

   Plasmid

   #179139

   Purpose

   Binder: SspB that carries two point mutations reducing homodimerization(Y11K/A15E, residue numbering from pdb: 1ou9). Binder binds to the 7 a.a. peptide SsrA (AANDENY).

   Depositor

   Klaus Hahn

   Article

   Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

   Insert

   SspB
   

   Use

   Tags

   FLAG and mCherry

   Expression

   Mammalian

   Mutation

   Y11K/A15E, residue numbering from pdb: 1ou9

   Promoter

   Available since

   Jan. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGFP-Kif5c

   Plasmid

   #71853

   Purpose

   mammalian expression of GFP-Kif5c

   Depositor

   Michelle Peckham

   Article

   Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

   Insert

   Kif5c (Kif5c Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Kif5c starts at AA3

   Promoter

   CMV

   Available since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. HSC1-HS4-D4Z4c-GiP

    Plasmid

    #58541

    Purpose

    Derived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoter

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Inserts

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    D4Z4 repeat, partial
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1-alpha

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-N1_MeCP2(WT)

    Plasmid

    #110186

    Purpose

    Expression of mouse MeCP2 (WT) in mammalian cells

    Depositor

    Adrian Bird

    Article

    Tillotson et al Nature. 2017 Oct 19;550(7676):398-401. d

    Insert

    MeCP2_e2 FL (mouse cDNA) (Mecp2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    May 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-TadA8e-PcrA M6

    Plasmid

    #221229

    Purpose

    Express HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    TadA8e-PcrA M6
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR223-BZLF1(Zta)

    Plasmid

    #195787

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BZLF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BZLF1 (BZLF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-SFFV-PYL1-sfGFP-Emerin

    Plasmid

    #121532

    Purpose

    expression of PYL-SfGFP-Emerin, lentivirus vector

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    Emerin (EMD Human)
    

    Use

    Lentiviral

    Tags

    PYL1-SFGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Feb. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. HiBiT-FZD8

    Plasmid

    #195846

    Purpose

    Expresses human FZD8 with an N-terminal HiBiT

    Depositor

    Gunnar Schulte

    Article

    Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

    Insert

    FZD8 (FZD8 Human)
    

    Use

    Tags

    HiBiT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR223-BILF1

    Plasmid

    #195797

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BILF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BILF1 (BILF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR223-BALF4

    Plasmid

    #195835

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BALF4

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BALF4 (BALF4 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPINS-USP21 (USP, aa 196-565)

    Plasmid

    #61585

    Purpose

    Expresses human USP21 (USP domain) in E. coli.

    Depositor

    David Komander

    Article

    Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

    Insert

    USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Deleted aa 1-195.

    Promoter

    T7

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENTR223-BLLF1(gp350)

    Plasmid

    #195837

    Purpose

    Gateway Entry Clone of Epstein-Barr virus BLLF1

    Depositor

    Eric Johannsen

    Article

    Calderwood et al Proc Natl Acad Sci U S A. 2007 May 1;104

    Insert

    BLLF1 (BLLF1 Epstein-Barr virus)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAV r3.45

    Plasmid

    #233684

    Purpose

    An evolved Adeno-associated viral variant, enhances gene delivery and gene targeting in neural stem cells

    Depositor

    David Schaffer

    Article

    Jang et al Mol Ther. 2011 Apr;19(4):667-75. doi: 10

    Insert

    Modified AAV2 Rep, Cap
    

    Use

    AAV

    Tags

    Expression

    Mutation

    insertion of LATQVGQKTA (where LA and A are linke…

    Promoter

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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