We narrowed to 31,812 results for: LIS;

1. pET21-pelB-LaRIgG-2

   Plasmid

   #228996

   Purpose

   For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaRIgG-2
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaGIgG-12

   Plasmid

   #228999

   Purpose

   For bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   LaGIgG-12
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-GST-3

   Plasmid

   #228985

   Purpose

   For bacterial expression of anti-GST nanobody GST-3, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

   Insert

   GST-3
   

   Tags

   6xHIS

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDB.His.MBP.3C CARD8 CARD WT

   Plasmid

   #164022

   Purpose

   Bacterial expression vector encoding a HRV 3C-cleavable His-MBP-tagged CARD8 UPA-CARD

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   His6-MBP-3C

   Expression

   Bacterial

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-Hsp-Fluc-PGK-Rluc-mCherry

   Plasmid

   #191863

   Purpose

   Heat inducible Fluc reporter with constitutive Rluc

   Depositor

   Yingxiao Wang

   Article

   Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

   Inserts

   Firefly luciferase Fluc
   Renilla luciferase Rluc
   

   Use

   Lentiviral

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Promoter

   HSPA7 promoter and PGK promoter

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV-mito-CAR-GECO1

   Plasmid

   #46022

   Purpose

   Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

   Depositor

   Robert Campbell

   Article

   Wu et al ACS Chem Neurosci. 2013 Mar 19.

   Insert

   CAR-GECO1
   

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

   Promoter

   CMV

   Available Since

   July 23, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pRK5-Ufm1 ∆C3

   Plasmid

   #133866

   Purpose

   Expression of human UFM1 mutant lacking the last three C-terminal residues

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   Ubiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-ADAR2-sesRNA-tTA2-W3SL

   Plasmid

   #192068

   Purpose

   Expression of ADAR2, sesRNA in mammalian cells with hSyn promoter, with tTA2 as efRNA and ADAR2 overexpression.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for mouse Ctip2
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW-Nb127D01-mCherry

   Plasmid

   #171573

   Purpose

   Expresses mCherry-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-mCherry) in fly cells

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   Nb127D01-mCherry (CXCR2 Human)
   

   Tags

   mCherry

   Expression

   Insect

   Promoter

   fly actin5C promoter

   Available Since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-EF1A-LivePAR(Y107A)-Hygro

    Plasmid

    #176073

    Purpose

    EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    LivePAR (Y107A)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Point mutation to convert Try107 to Ala

    Promoter

    EF1A

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-mNeptune2

    Plasmid

    #51309

    Purpose

    Expresses far-red fluorescent protein mNeptune2 in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mNeptune2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. MiniCoopR mitfa:BRAF

    Plasmid

    #118846

    Purpose

    Expresses human BRAF V600E mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    BRAF V600E (BRAF Human, Zebrafish)
    

    Use

    Zebrafish expression, tol2

    Mutation

    BRAF V600E

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC129: pAAV.CMV-CasRx mCh

    Plasmid

    #203442

    Purpose

    Plasmid expressing active RfxCas13d with mCherry reporter for investigating CasRx activity

    Depositor

    Guei-Sheung Liu

    Article

    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

    Insert

    RfxCas13d-T2A-mCherry
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. HMGN1_pLENTI-CAG-IRES-GFP

    Plasmid

    #176995

    Purpose

    Mammalian lentiviral expression vector encoding HMGN1

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    HMGN1 (HMGN1 Human)
    

    Use

    Lentiviral

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ-ABE-SpRYn-Act3.0

    Plasmid

    #178959

    Purpose

    It consists of a SpRY nickase (D10A) fused with an adenine deaminase (ABE8e) and MS2-SunTag-activators (ScFv-sfGFP-2xTAD), enabling simultaneous A to G conversion and gene activation.

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

    Insert

    ABE8e-SpRY-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV hUbC-dCas9-dMQ1-EGFG

    Plasmid

    #89794

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR and Lentiviral

    Tags

    3*FLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC; S317A, AGC-->GCC

    Promoter

    hUbC

    Available Since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CMV-eGFP-SV40p-BlasR

    Plasmid

    #228271

    Purpose

    pLenti-CMV-eGFP-SV40p-BlasR plasmid for ectopic expression of GFP

    Depositor

    Judy Lieberman

    Article

    Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST1374-scFv-APOBEC-UGI-GB1

    Plasmid

    #113029

    Purpose

    Expresses scFv-rAPOBEC1-UGI in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    scFv-rAPOBEC1-UGI-GB1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-mA3-BE3

    Plasmid

    #113419

    Purpose

    Expresses mA3-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    mA3-BE3 (Apobec3 Mouse, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-Tag2B-E2F3

    Plasmid

    #202522

    Purpose

    Expressing human E2F3 protein with FLAG-tag

    Depositor

    Ming Chen

    Article

    Wang et al J Clin Invest. 2023 Mar 16:e166647. doi:

    Insert

    E2F3 (E2F3 Human)
    

    Tags

    FLAG-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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