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pLVXCMV100-mNeonGreen-PBD

Plasmid

#241366

Purpose

Lentiviral expression of mNeonGreen-tagged PAK1 p21-binding domain (PBD)

Depositor

Gaudenz Danuser

Publication

Weiss et al Sci Signal. 2025 May 20;18(887):eadk0922

Insert

Pak1 fragment (PAK1 Human)

Use

Lentiviral

Tags

mNeonGreen

Mutation

aa 57-141

Promoter

Truncated CMV promoter

Available since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-NES-FRCaMPi

Plasmid

#232838

Purpose

AAV transfer plasmid for Syn-mediated expression of FRCaMPi

Depositor

Kiryl Piatkevich

Publication

Zhou et al PLoS Biol. 2025 Apr 29;23(4):e3003048. d

Insert

NES-SomaFRCaMPi

Use

AAV

Tags

nuclear export sequence

Expression

Mammalian

Promoter

Syn

Available since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pEHAC1- HA-BirA-Traf6

Plasmid

#232585

Purpose

Express BirA tagged to the N-terminus of TRAF6.

Depositor

Sagar Bhogaraju

Publication

Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BirA -TRAF6 (TRAF6 Human)

Tags

HA

Expression

Mammalian

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pFast-Flag-hRad9

Plasmid

#222468

Purpose

Expresses hRad9 in insect cells

Depositor

Aziz Sancar

Publication

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hRad9 (RAD9A Human)

Tags

Flag epitope

Expression

Insect

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)-T7-OmpA-MNaseA-6xHis

Plasmid

#214808

Purpose

Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

Depositor

Peter Sarin

Publication

Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

Insert

OmpA-Micrococcal nuclease A

Tags

6xhis

Expression

Bacterial

Promoter

T7 promoter

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pQE60 R130A wgMDH

Plasmid

#204266

Purpose

Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Publication

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R130A

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-tdTomato-WPRE

Plasmid

#202610

Purpose

Double floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Publication

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

tdTomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pegRNA-GBA-10

Plasmid

#199271

Purpose

pegRNA used to induce GBA (c. 1226 A > G) mutation

Depositor

Ting Zhou

Publication

Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GBA 1226AtoG pegRNA

Expression

Mammalian

Available since

April 13, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_KanR neo

Plasmid

#167885

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Publication

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HNRNPC-Halo

Plasmid

#175306

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Publication

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HNRNPC (HNRNPC Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pmiR-report-MYB-mut

Plasmid

#141115

Purpose

Reporter plasmid of mutant MYB in mammalian cells.

Depositor

Jianjun Chen

Publication

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYB (MYB Human)

Use

Luciferase

Expression

Mammalian

Mutation

A to T mutations at m6A sites

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET15-His-∆NGlpG

Plasmid

#155131

Purpose

Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

Depositor

Sinisa Urban

Publication

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Tags

His

Expression

Bacterial

Mutation

insert codes for GlpG residues 87-276

Promoter

T7

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp10-HF

Plasmid

#157708

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Publication

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp10-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC6A2

Plasmid

#131891

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Sijben et al Sci Rep. 2021 Jun 10;11(1):12290. doi: 1

Insert

SLC6A2 (SLC6A2 Human)

Expression

Mammalian

Available since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pSB97 - pL2_pVS1_2x35S::fLUC-I::tNOS

Plasmid

#123189

Purpose

binary plant vector for transient fLUC (with intron) expression

Depositor

Erin Cram, Carolyn Lee-Parsons

Publication

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::fLUC-I::tNOS

Use

Luciferase

Expression

Plant

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

Plasmid

#124860

Purpose

Mutagenesis of Slc17a6

Depositor

Larry Zweifel

Publication

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a6 (Slc17a6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Emerin

Plasmid

#120246

Purpose

Expresses V5-tagged Emerin in lentiviral vector

Depositor

Larry Gerace

Publication

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Emerin (Emd Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pOPS0359

Plasmid

#115508

Purpose

Reporter Plasmid. Taurine-inducible expression of GFP

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

sfGFP pOGG037, T-pharma pOGG003 assembled in pOGG024

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Taurine-inducible promoter of S. meliloti Promote…

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shLacZ-1735

Plasmid

#115454

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shLacZ-1735

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;lmo2CE1-P1Egfp

Plasmid

#90153

Purpose

contains lmo2 enhancer sequence, and lmo2 specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Publication

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

lmo2E1P1Egfp (lmo2 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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