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Showing: 6661 - 6680 of 15627 results

  8. pCRI001-pGEM-LS-Bar-PgpdA-dLbCas12a-VPR-Ttrpc-LS

    Plasmid
    #140193
    Purpose
    Chromosomal integration of PgpdA-dLbCas12a-VPR-TtrpC. Contains 1kb homology arms for Aspergillus nidulans and the bar gene for glufosinate selection. Filamentous fungi integrative vector.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Inserts
    dLbCas12a-VPR
    Bar
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
    Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
    Use
    CRISPR and Synthetic Biology; Fungal genome integ…
    Tags
    VPR (VP64-p65-Rta), NLS
    Mutation
    D832A DNAse deactivated
    Promoter
    PgpdA and PtrpC
    Available Since
    Sept. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    140193-plasmid-map-sequence-id-309648

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  9. pCRI011-pYFAC-pyroA-PgpdA-4xcrRNAarrayelcA-TtrpC

    Plasmid
    #140203
    Purpose
    Fungal vector to express an LbCas12a crRNA array targeted to Pelca, to be contransformed with pCRI009 containing PelcA-mCherry in a dLbCas12a-VPR strain as proof-of-concept of CRISPRa.
    Depositor
    Yit Heng Chooi
    Article
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Insert
    crRNA array elcA
    Use
    CRISPR and Synthetic Biology
    Promoter
    PgpdA
    Available Since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    140203-plasmid-map-sequence-id-310119

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  17. pSB63 - pL0_fLUC-I (CDS1)

    Plasmid
    #123180
    Purpose
    Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants
    Depositor
    Erin Cram, Carolyn Lee-Parsons
    Article
    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi
    Insert
    Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)
    Use
    Luciferase; Part for plant expression
    Expression
    Plant
    Mutation
    No BpiI and BsaI sites
    Available Since
    May 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    123180-plasmid-map-sequence-id-241283

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Showing: 6661 - 6680 of 15627 results