We narrowed to 14,099 results for: cas9

1. EGFP gRNA (BRDN0000562805)

   Plasmid

   #80035

   Purpose

   3rd generation lentiviral gRNA plasmid targeting EGFP

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   EGFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-hA3D-BE3

   Plasmid

   #113413

   Purpose

   Expresses hA3D-BE3 in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3D-BE3 (APOBEC3D Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. VRQR variant

   Plasmid

   #138562

   Purpose

   Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRQR
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335Q, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEL032

   Plasmid

   #137901

   Purpose

   STU2.0 LbCas12a for plant genome editing

   Depositor

   Yong Zhang

   Article

   Tang et al Plant Biotechnol J. 2019 Jul;17(7):1431-

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV ORANGE Gria1-HaloTag KI

   Plasmid

   #139655

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and HaloTag donor
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB2904(XI-3 MarkerFree)

   Plasmid

   #73276

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pORANGE GFP-Bsn KI

   Plasmid

   #139664

   Purpose

   Endogenous tagging of Bassoon: N-terminal (amino acid position: L7)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Tol2-LyzC-RacFRET-U6ac-ctrl guides

   Plasmid

   #168247

   Purpose

   "label active Rac; control for neutrophil-specific knockout"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   RacFRET
   

   Use

   Zebrafish expression

   Promoter

   LyzC

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB2909(XII-5 MarkerFree)

   Plasmid

   #73281

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-tLuc-mCherry

    Plasmid

    #107553

    Purpose

    tLuc-mCherry in AAV backbone

    Depositor

    Juan Belmonte

    Article

    Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

    Type

    Empty backbone

    Use

    AAV

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. 8xCTS1_deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA

    Plasmid

    #124537

    Purpose

    Cloning vector for tRNA-released synergistic activation mediator gRNA. BsmBI flanked placeholder for spacer cloning.

    Depositor

    Tudor Fulga

    Article

    Knapp et al Nat Commun. 2019 Apr 2;10(1):1490. doi:

    Insert

    deltaC55G-hutRNApro_BsmBIplch-SAMsgRNA_14nt Buffer_deltaC55G-hutRNApro
    

    Expression

    Mammalian

    Mutation

    d11-41 C55G, d11-41 C55G

    Promoter

    Minimal ADE

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORANGE GFP-Pclo KI

    Plasmid

    #139657

    Purpose

    Endogenous tagging of Piccolo: N-terminal (amino acid position: G2)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. dxCas(3.6)-VPR

    Plasmid

    #108388

    Purpose

    Mammalian expression vector for xCas9 3.6 with VP64-p65-Rta (VPR) fused to its C-terminus

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    dxCas(3.6)-VPR
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. PEA1

    Plasmid

    #176527

    Purpose

    Delivers all prime editing nickase components in a single plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CbH for Cas9, hU6 for gRNAs

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSP1594_2x_NLS

    Plasmid

    #110625

    Purpose

    Mammalian expression vector for Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9)

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    None
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXZX501

    Plasmid

    #169455

    Purpose

    pRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassette

    Depositor

    Jef Boeke

    Article

    Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

    Insert

    gRNA for MATa
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDG335

    Plasmid

    #100899

    Purpose

    SpCas9n (D10A Nickase mutant) with a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A mutant converts to Nickase

    Promoter

    CBh and U6

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-CMV-NLS-AcrIIA4

    Plasmid

    #113038

    Purpose

    AAV vector for expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    SV40 NLS-AcrIIA4
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXZX351

    Plasmid

    #169454

    Purpose

    pRS426-SNR52p-gRNA.MATα_option1-SUP4t; 20-bp gRNA sequence targeting MATα cassette

    Depositor

    Jef Boeke

    Article

    Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

    Insert

    gRNA for MATα
    

    Expression

    Yeast

    Promoter

    SNR52

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. ScFv-2ERT2-VPH

    Plasmid

    #120556

    Purpose

    Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1

    Depositor

    Yu Wang

    Article

    Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

    Insert

    scFvGCN4, GB1, ERT2, VP64, P65 and HSF1
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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