We narrowed to 20,660 results for: ACE
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Plasmid#207634PurposeA plasmid encoding photocaged SpyCatcher (pSC)-cUnaG, nUnaG-SpyTag (ST), and mCherry separated by P2A cleaving sequences for mammalian expression.DepositorInsertphotocaged SpyCatcher-cUnaG-P2A-nUnaG-SpyTag-P2A-mCh
ExpressionMammalianMutationAmber stop codon at SC's critical lysinePromoterCMVAvailable SinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHES839
Plasmid#87941PurposeConstitutively expresses estradiol-inducible synthetic transcriptional regulator in yeast. Transcriptional regulator activates transcription of promoters containing Gal4 binding sites.DepositorInsertGAL4 DBD - hER LBD - MSN2 AD (ESR1 Synthetic, Budding Yeast, Human)
UseSynthetic BiologyExpressionYeastPromoterADH1 (crippled)Available SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
460 p3E-2A-mCherrypA
Plasmid#195970PurposeGateway p3E 2A fluorophoreDepositorInsert2A-mCherry
UseOtherAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX330-LAMP1
Plasmid#207787PurposeExpresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.DepositorInsertsgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
UseCRISPRExpressionMammalianPromoterU6Available SinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRISPR-StAR4B (Lenti)
Plasmid#222691PurposeCRISPR-screeningDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianMutationPGK without BsmBI cutsiteAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
scOrange chromoprotein
Plasmid#117840PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coliDepositorInsertpromoter, RBS, scOrange
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
457 p3E-2A-EGFPpA
Plasmid#195967PurposeGateway p3E 2A fluorophoreDepositorInsert2A-EGFP
UseOtherAvailable SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAP05
Plasmid#186261PurposesfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoterDepositorInsertsExpressionBacterialPromoterBBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…Available SinceSept. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD7
Plasmid#195845PurposeExpresses human FZD7 with an N-terminal HiBiTDepositorAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCK007 pME KalTa4
Plasmid#195965PurposeGateway pME Gal4 variantDepositorInsertKalTA4 (Gal4)
UseOtherAvailable SinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-CreERT2 stuffer v3
Plasmid#158046Purposelenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9DepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 5, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
TagsAmilCP and RTXExpressionBacterialPromoterJ23100 promoterAvailable SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
amilGFP chromoprotein
Plasmid#117842PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilGFP
UseSynthetic Biology; Escherichia coliMutationBioBrick sites removedAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCK029 exorh:EGFP
Plasmid#195980PurposeExpression constructDepositorInsertexorh:EGFP
UseOtherAvailable SinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LBR pEGFP-N2 (646)
Plasmid#61996Purposemammalian expression of nuclear envelope transmembrane proteinDepositorAvailable SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
AMPK(T>A)-SPARK
Plasmid#226256Purposephospho-null mutant of AMPK-SPARKDepositorInsertAMPK(T>A)-SPARK
ExpressionMammalianMutationsubstituted Threonine 20 with AlanineAvailable SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
768 pME mApple-CAAX
Plasmid#195961PurposeGateway pME fluorophoreDepositorInsertmApple-CAAX (membrane)
UseOtherAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cyto-mTurq2-Apollo-NADP+
Plasmid#193301PurposeExpresses mTurq2-Apollo-NADP+ sensor localized to the cytoplasmDepositorInsertG6PD (G6PD Human)
TagsmTurqoise2ExpressionMammalianMutationS40A, R72Q, H201N, K205TPromoterT7Available SinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
764 pME nls-mApple
Plasmid#195962PurposeGateway pME fluorophoreDepositorInsertmApple nuclear
UseOtherAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-NBK-1
Plasmid#207639PurposeA plasmid for the expression of M. mazei pyrrolysine aminoacyl-tRNA synthetase/tRNA pair (PylRS/tRNAPyl) in E. Coli.DepositorInsertPyl-tRNACUA and 2x pyrrolysyl-tRNA synthetase (MmPylRS)
ExpressionBacterialMutationThe evolved synthetase NBK-1 contains mutations Y…PromoteraraBADAvailable SinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only