We narrowed to 13,416 results for: sequence
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Plasmid#114403PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human LMNB1DepositorInsertLMNB1 Homology Arms with mTagRFPT-linker (LMNB1 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available SinceSept. 4, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGG-GOI-DE-TurboID-GFP
Plasmid#209389PurposeGolden Gate compatible plasmid for TurboID-based proximity labelling experiment in plantaDepositorTypeEmpty backboneTagsGFPExpressionPlantMutationTurboID gene sequence C249AAvailable SinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDe-CAS9
Plasmid#61433PurposeBinary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequenceDepositorInsertCas9
UseCRISPRExpressionPlantPromoterPcUBI4-2Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB-BbsI-sgRNA
Plasmid#203359PurposeContains BbsI cut sites for placement of user-specified sgRNA target sequences with Sleeping Beauty backboneDepositorInsertBbsI Cut Sites
Available SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Calreticulin-SuperNova-KDEL
Plasmid#131857PurposeExpresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signalDepositorInsertSuperNova
TagsKDEL ER-retention signal and Signal sequence of h…ExpressionMammalianPromoterCMVAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQC-FsCFP-mCherryFP
Plasmid#154836PurposeFrame-shift fluorescent reporter for genomic DNA editingDepositorInserttargeting sequence of VEGF
UseRetroviralExpressionMammalianAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBigT
Plasmid#21270PurposeUsed for making transgenic mice. Contains a loxP-flanked cassette with a PGK-neo selectable marker and a tpA transcriptional stop sequence.DepositorTypeEmpty backboneUseMouse TargetingAvailable SinceJune 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
Multi-frame tag
Plasmid#128610PurposeIn the Multi-frame tag, HIV-1 frameshift sequence is upstream of FLAG epitopes in the 0 frame are separated from one another by SunTag epitopes in the -1 frame.DepositorInsertMF tag
ExpressionMammalianAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS sfGFP
Plasmid#154462PurposepET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-hrDNA-12.0
Plasmid#206320PurposeA plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)DepositorInsertIntergenic spacer of human rDNA repeat
UseCloningAvailable SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-AcHELIX-KanR (CJT96)
Plasmid#181794PurposepDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xIRS luciferase
Plasmid#13511DepositorAvailable SinceDec. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pHBS1147 GFP-TDP43
Plasmid#118797PurposeTo test the effect of sequence on nuclear body formation and splicing of full-length TDP43DepositorInsertTARDBP (TARDBP Human)
UseLentiviralAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB5319
Plasmid#204829PurposeAn integrating plasmid containing both the inducible enotetSW1 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW1 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
smHA multi-frame tag
Plasmid#128611Purposea spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.DepositorInsertsmHA MF tag
ExpressionMammalianAvailable SinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
phNFL
Plasmid#132589PurposeExpresses human NFLDepositorAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSC-GFP-NES-EGFP
Plasmid#221810PurposeLentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).DepositorInsertGFP-NES-EGFP
UseLentiviralTagsNES (internal tag)ExpressionMammalianPromoterCMVAvailable SinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD24-sfGFPx2
Plasmid#51559PurposeSuperfolder GFP ORF cloned into pBAD24 for expression in E. coli. It contains additional aminoacids in the N and C terminus (polilinker sequences for cloning proteins in frame with GFP)DepositorInsertsuperfolder GFP
TagsGLESTCRHASLAVLADERRFSA and MARARAExpressionBacterialMutationContains additonal aa in the N and C terminus (in…Available SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATG16L1 HRD
Plasmid#207537PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG16L1 locus in human cellsDepositorInsertHaloTag with internal PuroR cassette flanked by human ATG16L1 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-ATG9A HRD
Plasmid#203222PurposeHomologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous ATG9A locus in human cellsDepositorInsertHaloTag flanked by human ATG9 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only