We narrowed to 7,293 results for: Ank

1. pME-MCS (JDW 455)

   Plasmid

   #229832

   Purpose

   A gateway compatible middle entry clone containing an MCS (mutiple cloning site).

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   attL1/L2 flanked MCS
   

   Use

   Gateway entry clone

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-mStayGold-Puro-H2BC11-MMEJ

   Plasmid

   #227333

   Purpose

   MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-C-Solo-miRFP670nano3-H2BC11

   Plasmid

   #227331

   Purpose

   Donor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a miRFP670nano3 Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-Solo-mStayGold-H2BC11

   Plasmid

   #227330

   Purpose

   Donor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mStayGold Tag (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-C-mStayGold-EFS-Puro-PLK4

   Plasmid

   #227311

   Purpose

   Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. qTAG-N-Solo-mStayGold-PCNT

   Plasmid

   #227285

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the PCNT locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PCNT (Addgene #227284)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   PCNT Homology Arms flanking a mStayGold Tag (PCNT Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLXB3o1_hygR-MAR10-New BeYDV geminino-SP-nano72VHH-IgG1-MAR10 (GB4954)

   Plasmid

   #225441

   Purpose

   Module for stable transformation of Geminino 1.0-n72_h_HC flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.

   Depositor

   Diego Orzaez

   Article

   Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

   Insert

   hygR:MAR10:New BeYDV geminino:SP-nano72VHH-IgG1:MAR10
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. qTAG-N-Solo-sTagRFP-TUBA1B

   Plasmid

   #207765

   Purpose

   Donor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBactin-AcGFP-CI

   Plasmid

   #196849

   Purpose

   Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   AcGFP
   

   Expression

   Mammalian

   Mutation

   None (wt)

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-BS-AtMIR390a-B/c

    Plasmid

    #199559

    Purpose

    GATEWAY-compatible entry vector

    Depositor

    Alberto Carbonell

    Article

    Cisneros et al Nucleic Acids Res. 2023 Oct 27;51(19):10

    Insert

    Basal stem of Arabidopsis MIR390a precursor including a chloramphenicol-ccdB cassette flanked by two inverted BsaI sites (MIR390a Mustard Weed)
    

    Use

    Gateway-compatible entry vector

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1

    Plasmid

    #196877

    Purpose

    Neuron-specific expression of mOrange2 reporter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    Quimeric CAG

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBactin-td-mCherry-CI

    Plasmid

    #196851

    Purpose

    Expression of a tandem (td) red fluorescent reporter mCherry driven by the strong Beta-actin promoter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    td-mCherry
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBetaActin-KIF1aMotor-mScarlet-FKBP

    Plasmid

    #191336

    Purpose

    Expresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    KIF1aMotor-mScarlet-FKBP (Kif1a Mouse)
    

    Expression

    Mammalian

    Mutation

    motor domain aa 1-405

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. YW347_Ptbb2UTR-loxP-RFP-miniMOS

    Plasmid

    #196064

    Purpose

    a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

    Depositor

    Wenxing Yang

    Article

    Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

    Inserts

    myo2p driven tagRFP
    NeoR
    Minimal Mos1
    

    Expression

    Worm

    Promoter

    myo-2

    Available Since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBetaActin-FKBP-mScarlet-KIFC1motorN593K

    Plasmid

    #191335

    Purpose

    Expresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    FKBP-mScarlet-KIFC1N593K (KIFC1 Human)
    

    Expression

    Mammalian

    Mutation

    motor domain aa 125-673; N593K

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. attB53_SNCB-_attB53

    Plasmid

    #183615

    Purpose

    RMCE vector to shuttle puromycin resistance (+), anti-GFP SynNotch receptor (+), tagBFP (-) and TRE-mCherry (-) cassettes into attP50-flanked landing pad (Addgene 183609). (+) = sense (-) = antisense.

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Inserts

    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    

    Use

    Rmce

    Tags

    Myc and human CD8a signal sequence

    Expression

    Mammalian

    Available Since

    Sept. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. DHN_VrDHN1a_mCh-2xFKBP (pBS1117)

    Plasmid

    #185312

    Purpose

    For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DHN_VrDHN1a
    

    Expression

    Mammalian

    Available Since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST_DHN_VrDHN1a (or YSK2) (pBS0807)

    Plasmid

    #185266

    Purpose

    For the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a (or YSK2). This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    DHN_VrDHN1a (or YSK2)
    

    Expression

    Mammalian

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA-Prom1 D-4

    Plasmid

    #184016

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 651 to 665.

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 SV8(-Ex19a) D-4 (Prom1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of exon 19a (amino acids 696-701), and d…

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-Prom1 D-6

    Plasmid

    #184018

    Purpose

    Mammalian Flag tagged expression clone for the mouse Prom1 splicing variant SV8 (GenBank accession BC028286). Deletion of exon 19a (amino acids 696-701), and deletion of amino acids 631 to 645.

    Depositor

    Peter Stoilov

    Article

    Matalkah et al PLoS One. 2022 Oct 10;17(10):e0274958. d

    Insert

    Prom1 SV8(-Ex19a) D-6 (Prom1 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Deletion of exon 19a (amino acids 696-701), and d…

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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