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pMCh-1548

Plasmid

#82435

Purpose

Plasmid for expression of HA-tagged Nematostella vectensis AGO2 in mammalian cells

Depositor

Marina Chekulaeva

Publication

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

nvAGO2

Tags

HA

Expression

Mammalian

Promoter

EF1A

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Vimentin-7

Plasmid

#55099

Purpose

Localization: Intermediate Filaments, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

NM_003380.3 (VIM Human)

Tags

mCherry-miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
1132 pME-Lin28

Plasmid

#49009

Purpose

full length coding sequence for zebrafish lin28 gene in gateway middle entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

lin-28 homolog A (lin28ab Zebrafish)

Use

Gateway middle entry

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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666 pME-Lnx-1

Plasmid

#49006

Purpose

full length coding sequence for zebrafish lnx1 gene in gateway middle entry vector

Depositor

Nathan Lawson

Publication

Moore et al Dev Biol. 2013 Sep 11. pii: S0012-1606(1

Insert

ligand of numb-protein X 2b (lnx2b Zebrafish)

Use

Gateway middle entry

Available since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2_tRFP-T-vgll4b

Plasmid

#254506

Purpose

tagRFP-T tagged zebrafish vgll4b. For in vitro transcription (SP6) and RNA injection

Depositor

Virginie Lecaudey

Publication

Lardennois et al bioRxiv 2025.05.08.652796

Insert

vgll4b (vgll4 Zebrafish)

Use

Zebrafish expression

Tags

tagRFP-T

Promoter

SP6

Available since

Sept. 2, 2026

Availability

Academic Institutions and Nonprofits only

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16_GP_Vps4p_L7C_A43C

Plasmid

#203356

Purpose

Vps4 with stabilized MIT domain and 16aa GlyPro N-terminal extension

Depositor

Chris Hill

Publication

Wienkers et al Sci Rep. 2025 Jul 11;15(1):25024. doi: 1

Insert

Vps4p

Tags

6x-HIS-TEV and Gly Pro linker (16aa)

Expression

Bacterial

Mutation

L7C and A43C

Promoter

LacO

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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2xPeptide 8_Vps4p_L7C_A43C

Plasmid

#203358

Purpose

Vps4 with stabilized MIT domain with an N-terminal extension of 16 binding amino acids

Depositor

Chris Hill

Publication

Wienkers et al Sci Rep. 2025 Jul 11;15(1):25024. doi: 1

Insert

Vps4p

Tags

2xPeptide 8 and 6x-HIS-TEV

Expression

Bacterial

Mutation

L7C and A43C

Promoter

LacO

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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Vps4p_GP_linker

Plasmid

#203361

Purpose

Vps4 with GlyPro linker between MIT domain and Large ATPase Domain

Depositor

Chris Hill

Publication

Wienkers et al Sci Rep. 2025 Jul 11;15(1):25024. doi: 1

Insert

Vps4

Tags

6x-HIS-TEV

Expression

Bacterial

Mutation

Residues 83-111 are replaced with Gly Pro linker

Promoter

LacO

Available since

July 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSG1039-rA1-SaKKH_P2A_EGFP

Plasmid

#239462

Purpose

Cytosine base editor with codon-optimized S. aureus KKH Cas9 and rAPOBEC1 deaminase. EGFP can be used as a transfection marker.

Depositor

Alexis Komor

Insert

S.a. rA1 CBE

Use

CRISPR

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCas_4(I-U)/1_2_VB

Plasmid

#186445

Purpose

Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B). Cas4 domain swapped with type U-I Cas4 domain from G. sulfurreducens

Depositor

John van der Oost

Publication

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Mutation

Cas4 domain swapped with Cas4 domain from G. sulf…

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAF9D94 (pAF10C6)

Plasmid

#185848

Purpose

Expressing artificial trans-repressor in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Publication

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

PHAC1>TetR>CYC8>PABF1

Expression

Yeast

Mutation

None

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

Plasmid

#185299

Purpose

Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_3

Expression

Mammalian

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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DHN_VrDHN1a_mCh-SspB (pBS1074)

Plasmid

#185298

Purpose

Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_26

Expression

Mammalian

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q19228_CAEEL_93-127 (pBS0735)

Plasmid

#185231

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_93-127. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_93-127

Expression

Mammalian

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q7JLY3_CAEEL_48-124 (pBS0738)

Plasmid

#185232

Purpose

For the mammalian expression of the C. elegans protein Q7JLY3_CAEEL_48-124. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q7JLY3_CAEEL_48-124

Expression

Mammalian

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q19228_CAEEL_51-127_noLink (pBS0733)

Plasmid

#185229

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-127_noLink. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_51-127_noLink

Expression

Mammalian

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_H2FLK5_CAEEL_199-435 (pBS0696)

Plasmid

#185215

Purpose

For the mammalian expression of the C. elegans protein H2FLK5_CAEEL_199-435. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H2FLK5_CAEEL_199-435

Expression

Mammalian

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_H2FLK5_CAEEL_542-800 (pBS0695)

Plasmid

#185214

Purpose

For the mammalian expression of the C. elegans protein H2FLK5_CAEEL_542-800. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H2FLK5_CAEEL_542-800

Expression

Mammalian

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E4_80-255 (pBS0647)

Plasmid

#185192

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E4_80-255. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E4_80-255

Expression

Mammalian

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_E3_80-255 (pBS0646)

Plasmid

#185191

Purpose

For the mammalian expression of the human protein APOE_HUMAN_E3_80-255. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Publication

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_E3_80-255

Expression

Mammalian

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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