We narrowed to 14,587 results for: Cas9

Showing: 6761 - 6780 of 14587 results

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pCR1446

Plasmid

#111129

Purpose

Expresses FANCF

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCF (FANCF Human)

Use

Lentiviral

Tags

HA

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Article

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pEasyG1_nat

Plasmid

#184911

Purpose

Template to generate via PCR a single gRNA for expression in S. cerevisiae

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG3_mic

Plasmid

#184920

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

Plasmid

#162164

Purpose

Selectable homology donor template for Aag2 cell AGO1

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

RFP
3xFLAG

Use

CRISPR and Cre/Lox; Homology donor template

Tags

AGO1 homology and loxP

Expression

Insect

Promoter

Ae. aegypti PUb and endogenous AGO1

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Neo-EF1A>Tet3G

Plasmid

#184379

Purpose

The Tet3G gene is expressed under a constitutive EF1-alpha promoter. This protein binds a TRE3G promoter to activate gene transcription only in the presence of tetracycline or its analogs (e.g. doxycycline)

Depositor

Ori Bar-Nur

Article

Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

Insert

Tet3G

Use

Lentiviral

Available Since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) ACTB

Plasmid

#74705

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA

Use

CRISPR

Expression

Mammalian

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV_K700E_hSF3B1

Plasmid

#176591

Purpose

Adenoviral construct to change K to E at 700th amino acid (K700E) in human SF3B1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

SF3B1 (SF3B1 Human)

Use

AAV

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pEJS2712-CMV-eNme2-C-ABE8e-i1-L10

Plasmid

#228733

Purpose

Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

Domain inlaid eNme-C nCas9 (D16A)-i1-L10

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-SlugABE-NNG-P2A-puro

Plasmid

#214354

Purpose

Expresses SlugABE-NNG and puromycin resistance genes

Depositor

Yongming Wang

Article

Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

Insert

SlugABE-NNG and puromycin resistance genes

Expression

Mammalian

Mutation

Q782R/S888R/L906R/N984S/E1012K/K1016I

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_S34F_U2AF1

Plasmid

#176592

Purpose

Adenoviral construct to change S to F at 34th amino acid (S34F) in human U2AF1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

U2AF1 (U2AF1 Human)

Use

AAV

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #2

Plasmid

#139658

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere

Plasmid

#85715

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

telomere sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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ACTB-PQR-RFPnols-PQR-loxP-Zeo-loxP

Plasmid

#121448

Purpose

To make a stable human recombinant cell line; see right thumbnail map image for PQR annotations

Depositor

Brian Chen

Article

Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

Insert

RFPnols-PQR-ZeocinR (ACTB Human)

Use

CRISPR

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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p3015b

Plasmid

#209317

Purpose

Expresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.

Depositor

Thomas Hooven

Article

Gopalakrishna et al Commun Biol. 2023 Jun 9;6(1):620. doi: 1

Insert

Group B Streptococcus-compatible single guide RNA

Expression

Bacterial

Promoter

Xyl/tet promoter that is constitutively active

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEasyG2_mic

Plasmid

#184916

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSPneogRNA241510+MT

Plasmid

#84292

Purpose

Express LdPBK_241510.1 and LdMT targeting gRNAs simutaneously

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2017 Jan 18;2(1). pii: e00340-1

Insert

LdBPK_241510.1 targeting gRNA and LdMT targeting gRNA

Use

CRISPR; Leishmania donovani

Promoter

L. donovani ribosome RNA promoter

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pTPH413

Plasmid

#185728

Purpose

Bacterial PAM profiling of adenine base editor variants

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Inserts

ecTadA(8e)-neNme2-C
Nme2Cas9 sgRNA

Use

CRISPR

Expression

Bacterial

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

Plasmid

#216421

Purpose

Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

Depositor

Ellen Bushell

Article

Jonsdottir et al bioRxiv 2024.04.20.590404

Type

Empty backbone

Use

CRISPR; Expression in plasmodium berghei

Tags

3xmyc

Expression

Bacterial

Available Since

April 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCR1306

Plasmid

#111128

Purpose

Expresses FANCE.

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCE (FANCE Human)

Use

Lentiviral

Tags

HA

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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