We narrowed to 16,623 results for: puromycin

1. MBC1-PuroIII

   Plasmid

   #153419

   Purpose

   MSCV backbone with murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1) for expression of TCR genes

   Depositor

   Maki Nakayama

   Article

   Anderson et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

   Insert

   murine TCR-alpha signal peptide and murine TCR-beta1 constant region (TRBC1)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. tet-pLKO shMKP5.v2 puro

   Plasmid

   #87793

   Purpose

   Expresses an inducible short hairpin targeting human MKP5 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP5 (DUSP10 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE tight

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. tet-pLKO shMKP5.v1 puro

   Plasmid

   #87792

   Purpose

   Expresses an inducible short hairpin targeting human MKP5 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP5 (DUSP10 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE tight

   Available Since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sgRNA1_IL1RN

   Plasmid

   #64140

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-SLC35B2-sgRNA

   Plasmid

   #154860

   Purpose

   Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 sgRNA (SLC35B2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSMP-Bmi1_2

   Plasmid

   #36353

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   Bmi1 (BMI1 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pQsupR-Mi2b

   Plasmid

   #15379

   Depositor

   Stephen Smale

   Article

   Ramirez-Carrozzi et al Genes Dev. 2006 Feb 1. 20(3):282-96.

   Insert

   short hairpin RNA against mouse Mi-2 beta (Chd4 Mouse)
   

   Use

   RNAi and Retroviral

   Available Since

   April 18, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pBABEpuro-Flag-ZO-2

   Plasmid

   #27420

   Depositor

   Marius Sudol

   Article

   Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

   Insert

   Zonula Occludens 2 (TJP2 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

9. MSCV Puro SCRIB P305L

   Plasmid

   #88887

   Purpose

   SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

   Depositor

   Senthil Muthuswamy

   Article

   Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

   Insert

   SCRIB (SCRIB Human)
   

   Use

   Retroviral

   Mutation

   Proline to Leucine mutation @ 305th amino acid

   Promoter

   MSCV promoter

   Available Since

   March 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSBbiRP Hu Full Length CD142 (Tissue Factor; F3)

    Plasmid

    #183254

    Purpose

    Expression of full length human tissue factor (CD142; F3) in a Sleeping Beauty transposon vector

    Depositor

    Alexander McLellan

    Article

    Muhsin-Sharafaldine et al Oncotarget. 2016 Aug 30;7(35):56279-5629

    Insert

    Tissue factor (F3 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. HA-VHL Y112N-pBabe-puro

    Plasmid

    #19238

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 112 to Asparagin

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. MO70-hJunctate

    Plasmid

    #79596

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

    Insert

    junctate (ASPH Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

13. HA-VHL Y112H-pBabe-puro

    Plasmid

    #19237

    Depositor

    William Kaelin

    Article

    Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

    Insert

    von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    changed Tyrosin 112 to Histidin

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBabe puro-HA-PIK3CA(H1047R;G320E)

    Plasmid

    #33254

    Depositor

    Thomas Roberts

    Article

    Ilic et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Retroviral

    Tags

    Hemagglutinin (HA)

    Expression

    Mammalian

    Mutation

    changed histidine 1047 to arginine and glycine 32…

    Promoter

    SV40

    Available Since

    Jan. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pX459-HypaCas9-mR2-RHOA_sgRNA

    Plasmid

    #183877

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    RHOA sgRNA spacer (RHOA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHDR-eGFP (pXK-1647)

    Plasmid

    #208824

    Purpose

    The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR

    Depositor

    Kun Xu

    Article

    Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. tet-pLKO shMKP1.v2 puro

    Plasmid

    #87791

    Purpose

    Expresses an inducible short hairpin targeting human MKP1 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP1 (DUSP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE tight

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LENTICRISPR-PGAM1_sgRNA2

    Plasmid

    #201615

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PGAM1 (PGAM1 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBMN_HA-TBK1 (E696K)

    Plasmid

    #199207

    Purpose

    Used for the expression of TBK1 carrying E696K mutation (SMC Internal No. 1998)

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    E696K

    Available Since

    May 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p4949 pLPCX-His-Xpress NLS hBrd4 CTD

    Plasmid

    #14459

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 CTD (BRD4 Human)
    

    Use

    Retroviral

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    amino acids 1047-1362 of Brd4 (the CTD). Function…

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only