We narrowed to 2,342 results for: puc19

1. pAW091

   Plasmid

   #113253

   Purpose

   pUC19 backbone with S. pyogenes CRISPR array

   Depositor

   Jennifer Doudna

   Article

   Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

   Insert

   S. pyogenes CRISPR array
   

   Use

   Integration substrate

   Tags

   Expression

   Mutation

   Promoter

   NA

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC-T7-J1~398-Luc

   Plasmid

   #208639

   Purpose

   for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19

   Depositor

   Quanfu Ma

   Insert

   Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
   

   Use

   Luciferase; In vitro transcription template

   Tags

   Firefly Luciferase (F-Luc and T7 promoter

   Expression

   Mutation

   nt 1~398, in frame with donstream F-Luc

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT440

   Plasmid

   #8609

   Depositor

   Robert Hartley

   Article

   Yazynin et al Gene 1996 Feb 22;169(1):131-2.

   Insert

   barnase, barstar
   

   Use

   Tags

   as pMT416

   Expression

   Bacterial

   Mutation

   V36 codon of barnase of pMT416 replaced by polyli…

   Promoter

   Available since

   Jan. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pSelAct-KO

   Plasmid

   #174374

   Purpose

   Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible

   Depositor

   Gitta Coaker

   Article

   Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

   Type

   Empty backbone

   Use

   Vector for 2-step knockout by homologous recombin…

   Tags

   Expression

   Mutation

   Promoter

   N/A

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSelAct-Express

   Plasmid

   #174375

   Purpose

   Plasmid for integrative expression, pUC19 backbone, Apr resistant casette, pCMP1::eGFP expression cassette, gateway compatible

   Depositor

   Gitta Coaker

   Article

   Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(

   Type

   Empty backbone

   Use

   Expression vector for targeted integration

   Tags

   Expression

   Mutation

   Promoter

   pCMP1

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHB1

   Plasmid

   #204668

   Purpose

   pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs

   Depositor

   Mark Mandel

   Article

   Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do

   Insert

   FRT-erm-FRT
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCVD432

   Plasmid

   #63755

   Purpose

   Probe fragment for EAggEC. ca 1 Kb Sau3a1 fragment from plasmid 17-2, cloned into the BamHI site of pUC19.

   Depositor

   James Kaper

   Article

   Baudry et al J Infect Dis. 1990 Jun;161(6):1249-51.

   Insert

   EAggEC
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pILGFP1DFB

   Plasmid

   #185871

   Purpose

   A parental plasmid for in vivo gene amplification at RPL25 locus

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSL1119 (pDonor)

   Plasmid

   #160732

   Purpose

   Mini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.

   Depositor

   Samuel H. Sternberg

   Article

   Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

   Insert

   VchCAST donor DNA (Mini-Tn)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   N/A

   Available since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC-T7-hTP53-P0~670-FLuc

    Plasmid

    #208640

    Purpose

    for in vitro transcription template preparation, insert T7 promoter, TP53 translation initiation site P0 to +670 and F-luc from pGL3-Basic, into pUC19. P0~670 is confusing, BLAST is recommended.

    Depositor

    Quanfu Ma

    Insert

    TP53, UTR and partial CDS (TP53 Human)
    

    Use

    Luciferase; In vitor transcription template

    Tags

    Firefly Luciferase (F-Luc) and T7 promoter

    Expression

    Mutation

    UTR and partial CDS in-frame with F-Luc

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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