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Showing: 1321 - 1360 of 21949 results

  2. pJK693

    Plasmid
    #72382
    Purpose
    Produces Escherichia coli cysteine desulfurase IscS with Ala260>Thr mutation, N-terminal His6-tag (H6EcIscS-A260T)
    Depositor
    T. J. Kappock
    Insert
    cysteine desulfurase (tRNA sulfurtransferase), PLP-dependent (iscS Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated alanine-260 to threonine
    Promoter
    T7
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72382-plasmid-map-sequence-id-134008

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  6. pJK652

    Plasmid
    #72470
    Purpose
    Produces Escherichia coli acetyl-CoA:oxalate CoA-transferase (yfdE) with N-terminal His6 tag, His233>Gly mutant (H6EcYfdE-H233G)
    Depositor
    T. J. Kappock
    Insert
    acetyl-CoA:oxalate CoA-transferase (yfdE Escherichia coli str. K-12 substr. MG1655)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutated histidine-233 to glycine
    Promoter
    T7
    Available since
    April 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    72470-plasmid-map-sequence-id-134018

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  12. CUP1-∆ssCPY*-mGFP in pRS315

    Plasmid
    #221158
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mGFP
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    221158-plasmid-map-sequence-id-450962

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  13. CUP1-∆ssCPY*-mScarletI in pRS315

    Plasmid
    #221159
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Use
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Promoter
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221159-plasmid-map-sequence-id-450960

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  14. PRS_8-GCaMP8m

    Plasmid
    #210400
    Purpose
    AAV-vector for fluorescent calcium measurements in the green spectrum from noradrenergic neurons
    Depositor
    Simon Wiegert
    Article
    Wissing et al bioRxiv 2022.01.22.477348
    Insert
    GCaMP8m
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRSx8
    Available since
    Sept. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    210400-plasmid-map-sequence-id-415628

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  15. pLenti-SFNp-mRFP1

    Plasmid
    #238473
    Purpose
    Expresses higher mRFP1 fluorescence when NFkB is more active. Cloned into pLenti backbone
    Depositor
    Christopher Nelson
    Insert
    SFNp-mRFP1
    Use
    Tags
    Expression
    Mutation
    Promoter
    SFNp
    Available since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238473-plasmid-map-sequence-id-472675

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  17. pLenti6.2_mCherry_Grx1_roGFP2

    Plasmid
    #155045
    Purpose
    Fluorescent redox reporter
    Depositor
    Vanessa LaPointe
    Insert
    Glutaredoxin-1 (GLRX Human)
    Use
    Lentiviral
    Tags
    roGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    155045-plasmid-map-sequence-id-310403

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  18. pGEX6P1-N-FLAG

    Plasmid
    #119759
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Use
    Tags
    FLAG and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119759-plasmid-map-sequence-id-233663

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  19. pGEX6P1-N-His

    Plasmid
    #119755
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal 10x His tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Use
    Tags
    10xHis and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119755-plasmid-map-sequence-id-233662

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  23. pGEX6P1-N-Strep2

    Plasmid
    #119758
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal Strep-II tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Use
    Tags
    GST and Strep-tag II fusion tag
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119758-plasmid-map-sequence-id-233666

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  25. GFP-A96 ELP

    Plasmid
    #67856
    Purpose
    GFP Fluorescent Elastin-like Polypeptide(VPGAG) with 96 ELP repeats containing Alanine guest residue
    Depositor
    Andrew MacKay
    Insert
    GFP fused to ELP (VPGAG) repeated 96 times
    Use
    Tags
    GFP fused to N-terminal of ELP
    Expression
    Mammalian
    Mutation
    Promoter
    T7
    Available since
    Oct. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    67856-plasmid-map-sequence-id-117813

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  29. pER-rxRFP1.4

    Plasmid
    #67842
    Purpose
    redox-sensitive red fluorescent protein rxRFP1.4, endoplasmic reticulum localized
    Depositor
    Huiwang Ai
    Article
    Fan et al Anal Bioanal Chem. 2016 Jan 12.
    Insert
    rxRFP1.4
    Use
    Tags
    ER localization, ER retention, and His Tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    67842-plasmid-map-sequence-id-244285

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  30. tdTomato-Beta-Catenin-20

    Plasmid
    #58071
    Purpose
    Localization: Tight Junctions, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    Beta-Catenin (Ctnnb1 Mouse)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 4, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. EBFP2-Beta-Catenin-20

    Plasmid
    #55235
    Purpose
    Localization: Tight Junctions, Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Insert
    Beta-Catenin (Ctnnb1 Mouse)
    Use
    Tags
    EBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. mCerulean-Beta-Catenin-20

    Plasmid
    #55352
    Purpose
    Localization: Tight Junctions, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    Beta-Catenin (Ctnnb1 Mouse)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    55352-plasmid-map-sequence-id-177099

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Showing: 1321 - 1360 of 21949 results