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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,709 results for: plat

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Showing: 661 - 680 of 8709 results

  2. pMA_Int

    Plasmid
    #110096
    Purpose
    E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Insert
    mycophage integrase
    Use
    Required for transformation of attb integrating v…
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110096-plasmid-map-sequence-id-211299

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  3. qTAG-AAVS1-Ef1a-Puro-moxGFP

    Plasmid
    #227273
    Purpose
    AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    AAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227273-plasmid-map-sequence-id-452031

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  4. ICAM2-bio-His

    Plasmid
    #51758
    Purpose
    Expresses full-length Intercellular adhesion molecule 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ICAM2 (ICAM2 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51758-plasmid-map-sequence-id-79347

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  5. pBZ210-pU6-sgBFP-An_CBh-sv40NLS-Cas9-Ec86RT-NLS-T2A-mCherry

    Plasmid
    #170185
    Purpose
    Expression vector for a sgRNA against BFP and spCas9-Ec86 RT fusion protein linked to mCherry via a T2A peptide. Donor template An was inserted in the Retron Ec86 msr-msd region by SpeI and AvrII.
    Depositor
    Hunter Fraser
    Article
    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris
    Inserts
    Ec86 RT
    Retron Ec86 msr-msd
    BFP-to-GFP conversion donor template An
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    CBh and hU6
    Available Since
    Jan. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    170185-plasmid-map-sequence-id-333571

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  8. pJAT30

    Plasmid
    #204294
    Purpose
    For marking CRISPR HDR integrant with DsRed expressed in flight muscles. Second U6 promoter for using two gRNAs.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-DsRed
    Use
    CRISPR
    Promoter
    MHC
    Available Since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204294-plasmid-map-sequence-id-405490

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  9. pcDNA3.1-GGamma4-GFP2

    Plasmid
    #166776
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GGamma4-GFP2 (GNG4 Human, Synthetic)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available Since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166776-plasmid-map-sequence-id-330999

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  10. ITGA5-bio-His

    Plasmid
    #51909
    Purpose
    Expresses full-length Integrin alpha-5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ITGA5 (ITGA5 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51909-plasmid-map-sequence-id-79891

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  11. ANGPT-bio-His

    Plasmid
    #53404
    Purpose
    Expresses full-length Angiopoietin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    ANGPT (ANGPT1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53404-plasmid-map-sequence-id-83180

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  16. AICSDP-57: SMC1A-mEGFP

    Plasmid
    #114406
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A
    Depositor
    Allen Institute for Cell Science
    Insert
    SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Sept. 4, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114406-plasmid-map-sequence-id-220833

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  17. TFORF2234

    Plasmid
    #143180
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    GATA6 (GATA6 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143180-plasmid-map-sequence-id-397848

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Showing: 661 - 680 of 8709 results