We narrowed to 1,966 results for: rigi
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Plasmid#193726PurposeExpresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselectionDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only
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pfNL43-dGPE-EGFP
Plasmid#36866DepositorInsertNL43-dGPE-EGFP
UseLentiviral and RetroviralExpressionMammalianMutationparts of Gag and Pol were deleted and EGFP insert…Available SinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRAC10A
Plasmid#193734PurposeExpresses deactivated-Cas9 (J23110) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertionDepositorInsertdCas9
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
C1m
Plasmid#120955PurposeMoClo golden gate assembly CD part for deGFP (deGFP3 with 4aa N-truncation, 9aa C-truncation, F64L, and C203T (numbered with original sequence); see https://www.fpbase.org/protein/degfp3/)DepositorInsertdeGFP
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOpen-RNAse H
Plasmid#165575PurposeEndonuclease that specifically degrades the RNA of RNA-DNA hybrids. Participates in DNA replication; helps to specify the origin of genomic replication.DepositorInsertRNAse H
UseSynthetic BiologyAvailable SinceAug. 24, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
V37m
Plasmid#121043PurposeMoClo golden gate assembly AE destination vector DVCK-AE-mScar (ColE1/kan resistance destination vector with constitutive mScarlet expressing for screening.DepositorInsertKan destination vector with ColE1 origin and mScarlet selection
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
mVenus-P75ECD-VfAU1-LOV_458
Plasmid#63634PurposeExpresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by mVenus, the extracellular and transmembrane domain of p75NTR and a restriction siteDepositorInsertmVenus-P75ECD-VfAU1-LOV
ExpressionMammalianAvailable SinceApril 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPCE
Plasmid#193712PurposeExpresses cytosine deaminase linked deactivated-Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselectionDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceFeb. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
V35m
Plasmid#121042PurposeMoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.DepositorInsertKan destination vector with ColE1 origin and GFP selection
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSAH031
Plasmid#90330PurposePromoterless iLOV gene for recombineering, flanked by BsmbI restriction sites.DepositorInsertiLOV
TagsNonePromoterNoneAvailable SinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
C83m
Plasmid#120994PurposeMoClo golden gate assembly CD part for pir (Initiation factor required for replication of R6K origin plasmids). Please see Supplemental Documents for annotated Genbank file.DepositorInsertpir protein required for R6K ori
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT7-NSP3SA11-F2A-EGFP
Plasmid#220808PurposeExpress separately the Rotavirus SA11 NSP3 protein and the original enhanced GFP (Yang et al., 1996)DepositorInsertRotavirus SA11 NSP3-F2A-EGFP
UseOtherAvailable SinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSK29
Plasmid#80779PurposeCloning vector with temperature sensitive origin of replication.DepositorTypeEmpty backboneAvailable SinceDec. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFAP-mNeonGreen
Plasmid#105713PurposeAAV packaging vector to express mNeonGreen in astrocytes.DepositorInsertmNeonGreen
UseAAVPromoterMinimal GFAP promoterAvailable SinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTSA29
Plasmid#80778PurposeCloning vector with temperature sensitive origin of replication.DepositorTypeEmpty backboneAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHflu1
Plasmid#203878PurposepSU20 with addition of CEN6-ARSH4-HIS3 cassette for S. cerevisiae and RK2 origin of conjugal transferDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS327
Plasmid#51787Purposemulticopy (YEp) vector with a LYS2 marker and a 2-μm origin of replicationDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
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mGeos-M-C1
Plasmid#54815PurposeLocalization: C1 Cloning Vector, Excitation: 503, Emission: 514, reversibly switches on/off with UV. (Originally reported as Excitation: 503 / 569, Emission: 514 / 581)DepositorTypeEmpty backboneTagsmGeos-MExpressionMammalianAvailable SinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDTA-5
Plasmid#128845PurposeGateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy numberDepositorTypeEmpty backboneUseMouse TargetingExpressionMammalianAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only