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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,552 results for: ANG

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Showing: 6781 - 6800 of 11552 results

  3. N295A (EcN), E166Q (EcN) MetNI LmH

    Plasmid
    #118269
    Purpose
    N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetI (metI Escherichia coli)
    MetN (E166Q ; N295A) (metN Escherichia coli)
    Tags
    6x His Tag
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…
    Promoter
    T7 Promoter
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118269-plasmid-map-sequence-id-228542

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  4. pJTB115

    Plasmid
    #248909
    Purpose
    Expresses yeast Smk1 and Ssp2 fused to glutathione-S-transferase in bacteria
    Depositor
    Edward Winter
    Article
    Tio et al Mol Cell Biol. 2017 May 2;37(10):e00607-
    Inserts
    SMK1 Mitogen-activated Protein Kinase (SMK1 Sequence is from the SK1 isolate of S. cerevisiae and will differ by 1 non-synonomous/several synonymous changes, Budding Yeast)
    Ssp2 (SSP2 Sequence is from the SK1 isolate of S. cerevisiae and may differ by synonymous changes from S288C form, Budding Yeast)
    Tags
    glutathione-S-transferase
    Expression
    Bacterial
    Mutation
    NdeI and NcoI sites were removed for cloning purp…
    Promoter
    T7
    Available Since
    Jan. 16, 2026
    Availability
    Academic Institutions and Nonprofits only
    248909-plasmid-map-sequence-id-497710

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  5. pVZ_PcoaT_SQE1_THAS1

    Plasmid
    #103859
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and THAS1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    thalianol synthase 1 (THAS1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    103859-plasmid-map-sequence-id-217087

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  6. pLV[Exp]-Bsd-CMV>hCDK11B D562A, G579S

    Plasmid
    #239218
    Purpose
    Expresses CDK11B with D562A kinase inactivating mutation and G579S resistance mutation in mammalian cell lines
    Depositor
    Jason Sheltzer
    Article
    Julian et al bioRxiv 2025.08.03.668359; doi: https://
    Insert
    cyclin dependent kinase 11B with D562A kinase inactivating mutation and G579S resistance mutation (CDK11B Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    changed Aspartic acid 562 to Alanine and changed …
    Available Since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    239218-plasmid-map-sequence-id-476822

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  7. pAAV-EGFP.T2A.NCLX.3'UTR

    Plasmid
    #181872
    Purpose
    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)
    Depositor
    Hilmar Bading
    Article
    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10
    Inserts
    solute carrier family 8 member B1 (Slc8b1 Mouse)
    EGFP
    3'UTR of solute carrier family 8 member B1
    Use
    AAV and Adenoviral
    Tags
    HA, T2A, and myc
    Expression
    Mammalian
    Mutation
    includes a large portion of the Slc8b1 3'UTR…
    Promoter
    synthetic hybrid CAG promoter
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    181872-plasmid-map-sequence-id-356018

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  8. pTH862-CEN-slowmaxRLuc/minCFLuc

    Plasmid
    #115371
    Purpose
    Expresses translation initiation-controlled Renilla luciferase and translation elongation-controlled firefly luciferase
    Depositor
    Tobias von der Haar
    Inserts
    Firefly Luciferase (codon minimised)
    Gcn4 5'-UTR + Renilla luciferase (codon optimised)
    Expression
    Yeast
    Mutation
    All codons have been changed for the most favoura…
    Promoter
    ADH1 and TDH3
    Available Since
    June 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    115371-plasmid-map-sequence-id-247306

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  9. N295A (EcN), M163A (EcI) MetNIQ in pBAD

    Plasmid
    #118261
    Purpose
    N295A Mutation of MetN and M163A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (M163A) (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118261-plasmid-map-sequence-id-228528

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  10. N295A (EcN), M107A (EcI) MetNIQ in pBAD

    Plasmid
    #118259
    Purpose
    N295A Mutation of MetN and M107A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (M107A) (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118259-plasmid-map-sequence-id-228500

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  11. N295A (EcN), F103A (EcI) MetNIQ in pBAD

    Plasmid
    #118258
    Purpose
    N295A Mutation of MetN and F103A in MetI in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (F103A) (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118258-plasmid-map-sequence-id-228498

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Showing: 6781 - 6800 of 11552 results