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pHACK-GAL4>QF2

Plasmid

#80275

Purpose

GAL4->QF2 HACK construct. Contains 5' and 3' GAL4 homology arms and gRNAs.

Depositor

Christopher Potter

Article

Lin et al Genetics. 2016 Jun 22. pii: genetics.116

Insert

QF2

Expression

Insect

Available Since

Aug. 2, 2016

Availability

Academic Institutions and Nonprofits only

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DVK_pBR322_EF

Plasmid

#217594

Purpose

Destination vector with kanamycin resistance and pBR322 origin of replication. Carries E-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-dCry-V5His

Plasmid

#136241

Purpose

Protein expression of D. melanogaster (Dm) cytochrome with V5 and His tags

Depositor

Aziz Sancar

Article

Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

Insert

DmCry

Tags

His and V5

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-6xHis-MBP-TEV site-TadA8e

Plasmid

#208319

Purpose

Overxpression MBP-TadA8e

Depositor

Weixin Tang

Article

Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

TadA8e

Expression

Bacterial

Mutation

mutations in TadA are described in the paper

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pMZ0002

Plasmid

#102239

Purpose

Expresses Chinese hamster NSF in bacteria

Depositor

Axel Brunger

Article

Zhao et al Nature. 2015 Feb 5;518(7537):61-7. doi:

Insert

N-ethylmaleimide sensitive factor

Tags

6x His tag

Expression

Bacterial

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDUP 4-1

Plasmid

#23022

Depositor

Douglas Black

Article

Modafferi et al Mol Cell Biol. 1997 Nov . 17(11):6537-45

Insert

c-src (Src Mouse)

Expression

Mammalian

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only

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p4027 GST-ubiquitin

Plasmid

#10861

Depositor

Peter Howley

Article

Scheffner et al Cell. 1993 Nov 5. 75(3):495-505.

Insert

ubiquitin (UBI4 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pJA252

Plasmid

#21512

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

pmex-5 (w/o ATG)

Use

Multisite gateway

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pIIIA/MS2-2

Plasmid

#220632

Purpose

For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

Depositor

Marvin Wickens

Article

Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

Type

Empty backbone

Expression

Yeast

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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DVC_pBR322_AF

Plasmid

#217598

Purpose

Destination vector with chloramphenicol resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Article

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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