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pILGFPY2A

Plasmid

#165066

Purpose

Characterisation of 2A-mediated bicistronic expression of Y-FAST and yEGFP

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>Y-FAST-ErB1.2A-yEGFP>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pILGFP104

Plasmid

#165059

Purpose

Integrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pJAID9L

Plasmid

#165052

Purpose

Integrative expression of yEGFP and rice auxin receptor gene OsTIR1. OsTIR1 is codon-optimised for expression in S. cerevisiae

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Expression

Yeast

Mutation

WT

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Aaw-Glucoamylase SP

Plasmid

#153497

Purpose

Aspergillus awamori Glucoamylase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Glucoamylase SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce- α Galactosidase SP

Plasmid

#153504

Purpose

α Galactosidase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

α Galactosidase SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Gga-Lysozym SP

Plasmid

#153505

Purpose

Chicken Lysozym Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Lysozym SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce-Prepro SP

Plasmid

#153491

Purpose

S. cerevisiae Prepro Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Prepro SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM9121_Sce-Invertase 2 SP

Plasmid

#153493

Purpose

S. cerevisiae Invertase Signal Peptide Donor Plasmid

Depositor

Sylvestre Marillonnet

Article

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Insert

Invertase 2 SP

Use

Synthetic Biology

Available Since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pC0.007

Plasmid

#119671

Purpose

Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

Pcpc560-Dx2

Use

Synthetic Biology

Mutation

bp 89 T to C, bp 187 C to A, Duplicated downstrea…

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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brr2-C520R pRS313

Plasmid

#111412

Purpose

brr2-C520R (endogenous promoter)

Depositor

Christine Guthrie

Article

Mayerle et al bioRxiv 2018. 354514

Insert

BRR2

Expression

Yeast

Mutation

C520R aa mutation

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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brr2-N1104A pRS313

Plasmid

#111414

Purpose

brr2-N1104A (endogenous promoter)

Depositor

Christine Guthrie

Article

Mayerle et al bioRxiv 2018. 354514

Insert

BRR2

Expression

Yeast

Mutation

N1104A aa mutation

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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brr2-R1107L pRS313

Plasmid

#111416

Purpose

brr2-R1107L (endogenous promoter)

Depositor

Christine Guthrie

Article

Mayerle et al bioRxiv 2018. 354514

Insert

BRR2

Expression

Yeast

Mutation

R1107L aa mutation

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-RFP-miR-30 backbone

Plasmid

#97147

Purpose

As a control of miRNA expression

Depositor

Qing Deng

Article

Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

Type

Empty backbone

Use

Zebrafish

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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P0050::kaiBC

Plasmid

#101828

Purpose

drives expression of kaiBC from an rpaA-independent promoter

Depositor

Erin O'Shea

Article

Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

Insert

kanamycin casette and p0050 promoter

Use

Cloning vector

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90285

Purpose

BB2 with split pyrG marker for homologous integration with linker AB (negative control)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90286

Purpose

BB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90287

Purpose

BB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8

Plasmid

#90288

Purpose

BB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)

Depositor

Michael Sauer

Article

Sarkari et al Bioresour Technol. 2017 May 4. pii: S096

Insert

pyrGtrunc

Expression

Bacterial

Mutation

truncated version of pyrG gene (sequence is from …

Available Since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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AAT_g1 gRNA

Plasmid

#86005

Purpose

plasmid vector encoding for U6-driven AAT_g1 gRNA

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

Insert

pU6-AAT_g1 sgRNA

Use

CRISPR

Expression

Mammalian

Promoter

pU6

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-FRB(KTF)-HA no-pA

Plasmid

#82596

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Mutation

W2101F

Promoter

None

Available Since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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