We narrowed to 16,623 results for: puromycin

1. HA-VHL Y98H-pBabe-puro

   Plasmid

   #19235

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   changed Tyrosin 98 to Histidin

   Available Since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA2_IL1RN

   Plasmid

   #64142

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2_IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA4_IL1RN

   Plasmid

   #64152

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA4_IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX459_HR_Prnp_3

   Plasmid

   #78623

   Purpose

   pX459 vector encoding SpCas9-2A-Puro and a chimeric guide RNA targeting Prnp coding sequence

   Depositor

   Walker Jackson

   Article

   Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

   Insert

   HR_Prnp_3 (Prnp Mouse)
   

   Use

   CRISPR and Mouse Targeting

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. p6896 PHAGE-P CMVT N-HA-hE6AP II wt

   Plasmid

   #37603

   Depositor

   Peter Howley

   Article

   Martinez-Noel et al Mol Cell Biol. 2012 May 29.

   Insert

   E6AP II wt (UBE3A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV tet regulatable

   Available Since

   Aug. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. lentiCRISPR.sgKras.9

   Plasmid

   #91894

   Purpose

   sgRNAs targeting mouse Kras. 3rd generation lentiviral backbone.

   Depositor

   Wen Xue

   Article

   Mou et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

   Insert

   Kras sgRNA
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ1371-sgITGB4-3

   Plasmid

   #121536

   Purpose

   sgITGB4-3 sequence: GAGGAGCGTAGGTCCTCGCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

   Depositor

   Ovijit Chaudhuri, Stanley Qi

   Article

   Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

   Insert

   sgITGB4-3
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   mouse U6

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAPM-D4 miR30-DNMT3a ts3

   Plasmid

   #115867

   Purpose

   DNMT3a knockdown

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

   Insert

   DNMT3a shRNA (DNMT3A Human)
   

   Use

   Lentiviral

   Promoter

   SFFV

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA-VHL 1-115-pBabe-puro

   Plasmid

   #19243

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   von Hippel-Lindau tumor suppressor (VHL) (VHL Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   deleted amino acids 116 - 213 (end)

   Available Since

   Oct. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSMP-Ehmt1_1

    Plasmid

    #36335

    Depositor

    George Daley

    Article

    Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

    Insert

    Ehmt1 (EHMT1 Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

    Plasmid

    #105025

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

    Plasmid

    #105026

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT2-7xTcf-NLS-ONL

    Plasmid

    #65713

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL
    

    Use

    Luciferase

    Available Since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe puro H-Ras 12V (37G)

    Plasmid

    #12589

    Depositor

    Channing Der

    Article

    McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

    Insert

    HRas 12V 37G (HRAS Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    12V, 37G

    Available Since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pLPC-Myc-TRFcT

    Plasmid

    #44573

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    chimeric TRF (TERF2, TERF1 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    TRF2 where TRFH domain (aa42-241) has been replac…

    Available Since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

16. attB‐mCh­‐Poly(A)-­PuroR

    Plasmid

    #65529

    Purpose

    PuroR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    attB-mCherry
    

    Use

    Mouse Targeting; Bxb1

    Expression

    Mammalian

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe JunD-Venus puro

    Plasmid

    #58496

    Purpose

    mammalian expression of Venus-tagged mouse JunD

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JunD (Jund Mouse)
    

    Tags

    Venus

    Expression

    Mammalian

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pAPM-D4 miR30-AGO3 ts3

    Plasmid

    #115858

    Purpose

    AGO3 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    AGO3 shRNA (AGO3 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAPM-D4 miR30-AGO3 ts2

    Plasmid

    #115857

    Purpose

    AGO3 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    AGO3 shRNA (AGO3 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ADCK4 gRNA (BRDN0001145019)

    Plasmid

    #76560

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADCK4

    Depositor

    John Doench, David Root

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADCK4
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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