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psd44-iGFP-MFGE8-long

Plasmid

#46847

Purpose

expression of MFGE8 in mammalian cells

Depositor

Agnese Mariotti

Article

Carrascosa et al Oncogene. 2012 Mar 22;31(12):1521-32. do

Insert

MFGE8 (Mfge8 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK promoter

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only
XE248 pCDNA3.1+zeo-VSV-KLHL12

Plasmid

#16761

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

pCDNA3.1+zeo-VSV-KLHL12 (KLHL12 Human)

Tags

VSV

Expression

Mammalian

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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pDIO-DSE-mCherry-PSE-MCS

Plasmid

#129669

Purpose

Backbone that can be used to generate AAV virus where, upon cre-specific recombination, mCherry and any shRNA can be simultaneously expressed

Depositor

Beatriz Rico

Article

Favuzzi et al Science. 2019 Jan 25;363(6425):413-417.

Type

Empty backbone

Use

AAV

Tags

mCherry

Promoter

EF1a

Available Since

Dec. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 - hSRSF6

Plasmid

#120419

Purpose

overexpression of human SRSF6

Depositor

Gillian Bates, Andreas Neueder

Article

Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

Insert

SRSF6 (SRSF6 Human)

Expression

Mammalian

Promoter

CMV immediate/early

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-SZT2

Plasmid

#87035

Purpose

mammalian expression of SZT2

Depositor

David Sabatini

Article

Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

Insert

SZT2 (SZT2 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2017

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1-P2A-ERG-IMPROVED

Plasmid

#154089

Purpose

Improved version to express RUNX1, P2A, ERG in mammalian cells.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene (RUNX1, ERG Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

Plasmid

#84246

Purpose

Expresses direct fusion VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sa dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pET28a-pro-sumo1

Plasmid

#25101

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

pro-SUMO1 (SUMO1 Human)

Tags

His

Expression

Bacterial

Mutation

Changed Serine 2 to Alanine

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only
TFORF2069

Plasmid

#141902

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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EF1a-mito-dsRED2

Plasmid

#174541

Purpose

mitochondria targeted dsRED expressed off of EF1a promoter

Depositor

Thomas Schwarz

Article

Falk et al J Cell Biol. 2026 Apr 6;225(4):e20250102

Insert

Mito

Use

Lentiviral

Tags

dsRED

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG mito-mEos2

Plasmid

#168515

Purpose

Expression of photoconvertible mEos2 targted to mitochondrial matrix to allow tracking of individual mitochondria and measurement of fission-fusion rate

Depositor

Franck Polleux

Article

Lewis TL et al Nat Commun. 2018 Nov 27;9(1):5008. doi:

Insert

mito-mEos2

Tags

mEos2

Expression

Mammalian

Promoter

CAG

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pwB2b

Plasmid

#32094

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Inserts

BKV VP1
BKV VP2

Expression

Mammalian

Mutation

codon modified

Available Since

Oct. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2D4

Plasmid

#15786

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2D4 (UBE2D4 Human)

Tags

His

Expression

Bacterial

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only
Flag-Paxillin

Plasmid

#15212

Depositor

Rick Horwitz

Article

Webb et al J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-

Insert

Paxillin (PXN Chicken)

Tags

Flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pGreen2x35SLUC

Plasmid

#108228

Purpose

Plant expression as translational fusions to FLUC with RLUC normalization

Depositor

Alejandro Ferrando

Article

Solé-Gil et al Methods Mol Biol. 2024;2722:79-87. doi:

Type

Empty backbone

Use

Luciferase

Tags

Firefly Luciferase

Expression

Plant

Promoter

2x35S

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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CFP-NS1

Plasmid

#85739

Purpose

expresses CFP-FLAG-tagged NS1 monobody.

Depositor

John O'Bryan

Article

Spencer-Smith et al Nat Chem Biol. 2016 Nov 7. doi: 10.1038/

Insert

FLAG-tagged NS1 monobody

Tags

ECFP and Flag

Expression

Mammalian

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pSMP-Suv39H1_1

Plasmid

#36342

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Suv39H1 (SUV39H1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
human p53-(1-320)

Plasmid

#24864

Depositor

Cheryl Arrowsmith

Article

Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-320

Available Since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-VRK1

Plasmid

#23496

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

VRK1 (VRK1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pSIN ReP5-GFP-GluR1

Plasmid

#24004

Depositor

Robert Malinow

Article

Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

Insert

GluR1 (Gria1 Rat)

Use

Sindbis virus production

Tags

GFP

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only