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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,486 results for: his

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Showing: 6821 - 6840 of 23486 results

  1. pJCC_162 SpCas9 KES(1107-1109)GG

    Plasmid
    #179526
    Purpose
    For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 KES(1107-1109)GG
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    replaced KES (residues 1107-1109) with GG
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179526-plasmid-map-sequence-id-351748

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  2. pJCC_163 SpCas9 K233A/K234A/K253A/K263A

    Plasmid
    #179527
    Purpose
    For bacterial expression of SpCas9 K233A/K234A/K253A/K263A (helix-rolling basic patch mutant) with an N-terminal His-MBP tag
    Depositor
    Jennifer Doudna
    Article
    Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-
    Insert
    SpCas9 K233A/K234A/K253A/K263A
    Tags
    10X His, TEV protease cleavage site, and maltose-…
    Expression
    Bacterial
    Mutation
    changed lysines 233, 234, 253, and 263 to alanine
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    179527-plasmid-map-sequence-id-351733

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  17. pQE81XN-mGold

    Plasmid
    #218959
    Purpose
    Expresses Hisx6-Tagged mGold in bacteria
    Depositor
    Benjamin Glick
    Insert
    mGold
    Tags
    6X-His
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Sept. 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    218959-plasmid-map-sequence-id-445675

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  18. pET21a-LIC

    Plasmid
    #62303
    Purpose
    E. coli expression vector with N-terminal Hexa-HIS and TEV cleavage
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62303-plasmid-map-sequence-id-102191

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  19. pQE81XN-msYFP

    Plasmid
    #218960
    Purpose
    Expresses Hisx6-Tagged msYFP in bacteria
    Depositor
    Benjamin Glick
    Insert
    msYFP
    Tags
    6X-His
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218960-plasmid-map-sequence-id-446223

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  20. pLIC-SGC1

    Plasmid
    #39187
    Purpose
    SGC Empty backbone for bacterial expression
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
    39187-plasmid-map-sequence-id-160533

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Showing: 6821 - 6840 of 23486 results