We narrowed to 10,685 results for: plasmids 101

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pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG

Plasmid

#127325

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG

Plasmid

#127324

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAG

Plasmid

#127327

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAG

Plasmid

#127326

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2019

Availability

Academic Institutions and Nonprofits only

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gh25

Plasmid

#106706

Purpose

expression of gRNA targeting POMGNT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

POMGNT1 (POMGNT1 Human)

Expression

Mammalian

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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gh140

Plasmid

#106810

Purpose

expression of gRNA targeting GBGT1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GBGT1 (GBGT1 Human)

Expression

Mammalian

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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gh235

Plasmid

#106849

Purpose

expression of gRNA targeting DPY19L2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

DPY19L2 (DPY19L2 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh182

Plasmid

#106843

Purpose

expression of gRNA targeting GLT6D1

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GLT6D1 (GLT6D1 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh169

Plasmid

#106831

Purpose

expression of gRNA targeting ABO

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

ABO (ABO Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh166

Plasmid

#106817

Purpose

expression of gRNA targeting A3GALT2

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

A3GALT2 (A3GALT2P Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh34

Plasmid

#106711

Purpose

expression of gRNA targeting B4GALNT4

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

B4GALNT4 (B4GALNT4 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_4

Plasmid

#73536

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
FUP95Q15ADSKGW (D7)

Plasmid

#74030

Purpose

lentiviral expression of Psd95-Q15A deltaSK-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Xu et al Neuron. 2008 Jan 24;57(2):248-62. doi: 1

Insert

Psd95 (DLG4 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

Q15A, delta SH3-GK (deletion of the fragment betw…

Promoter

hUbC

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
Liu Lab MusCK Library A

Pooled Library

#174196

Purpose

MusCK library contains 23,622 unique sgRNAs targeting 4,787 gene locations in the genome. Sub-library MusCK A contains 5 gRNAs per gene.

Depositor

Xiaole Shirley Liu

Article

Wang et al Cell. 2021 Oct 14;184(21):5357-5374.e22.

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only
Liu Lab MusCK Library B

Pooled Library

#174197

Purpose

MusCK library contains 23,622 unique sgRNAs targeting 4,787 gene locations in the genome. Sub-library MusCK M_b contains 5 gRNAs per gene.

Depositor

Xiaole Shirley Liu

Article

Wang et al Cell. 2021 Oct 14;184(21):5357-5374.e22.

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only
CoV2-Spike-D614G-Gamma

Plasmid

#177963

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (Gamma: L13F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (Gamma: L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I, V1176F) (S )

Expression

Mammalian

Mutation

L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N50…

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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gh92

Plasmid

#106763

Purpose

expression of gRNA targeting FUT8

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

FUT8 (FUT8 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgSlc17a6

Plasmid

#124847

Purpose

Mutagenesis of Slc17a6 with SauCas9

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Slc17a6 gRNA (Slc17a6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

Plasmid

#197885

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD47-T2A-GFP

Plasmid

#205449

Purpose

lentiviral plasmid for expression of mouse CD47

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD47 (Cd47 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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