We narrowed to 16,623 results for: puromycin

1. pLenti PGK Puro GFP-GABARAPL1 G116A

   Plasmid

   #123245

   Purpose

   Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.

   Depositor

   Robin Ketteler

   Article

   Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

   Insert

   Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Glycine 116 to Alanine

   Promoter

   PGK

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSMP-DNMT1_2

   Plasmid

   #36383

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   DNMT1 (DNMT1 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   June 22, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pLenti_dNmeCas9

   Plasmid

   #120077

   Purpose

   dNmeCas9 with no sgRNA cassette lenti construct

   Depositor

   Erik Sontheimer

   Article

   Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

   Insert

   dNmeCas9
   

   Use

   Lentiviral

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABEpuro-ARAF_S214C

   Plasmid

   #51077

   Purpose

   mammalian expression of ARAF S214C

   Depositor

   Matthew Meyerson

   Article

   Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

   Insert

   ARAF (ARAF Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   S214C

   Available Since

   Feb. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSL011_AAVS1-puro-9xTetO-pEF-H2B-mCitrine-pRSV-H2B-mCherry

   Plasmid

   #179428

   Purpose

   Dual fluorescent reporter construct with no spacer, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   TRE-cit-(nospacer)-mch
   

   Expression

   Mammalian

   Promoter

   pEF alpha, pRSV

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSMP-MeCP2_1

   Plasmid

   #36377

   Depositor

   George Daley

   Article

   Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

   Insert

   MeCP2 (MECP2 Human)
   

   Use

   RNAi and Retroviral

   Expression

   Mammalian

   Available Since

   May 2, 2012

   Availability

   Academic Institutions and Nonprofits only

7. DsRed +9e

   Plasmid

   #191766

   Purpose

   Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

   Depositor

   Daniel Gerlich

   Article

   Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

   Insert

   DsRed
   

   Tags

   N terminal fusion of positively charged polypetod…

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDP1.2

   Plasmid

   #171092

   Purpose

   Expresses cell cycle-dependent OsTIR1 fused with mEmerald and Geminin (1-110). AKA ROLECCS G2(allows integration into AAVS1 safe-harbor)

   Depositor

   Dario Palmieri

   Article

   Capece et al Cell Death Differ. 2023 Aug 3. doi: 10.1

   Insert

   AAVS1-CMV-OsTIR1-mEmerald-Geminin-Puro-AAVS1
   

   Use

   Synthetic Biology

   Tags

   Emerald, Geminin

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_mU6-sgNGN3_hU6-sgRNA_hUbC-PuroR-P2A-GFP

   Plasmid

   #162336

   Purpose

   Lentiviral expression of sgNGN3 paired with a second S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

   Depositor

   Charles Gersbach

   Article

   Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

   Insert

   S. pyogenes sgRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6; mU6

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK233 (AAVS1 CMV-AtAFB2)

    Plasmid

    #140537

    Purpose

    AAVS1 CMV-AtAFB2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-AtAFB2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1-puro U6 sgRNA SOX17 -91

    Plasmid

    #50923

    Purpose

    U6 driven sgRNA targeting Sox17 -91 bp from TSS

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Sox17 -91 sgRNA (SOX17 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-UBE3B-BirA-Puro

    Plasmid

    #107352

    Purpose

    Expresses UBE3B fused to BirA in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3B-BirA (UBE3B Human)
    

    Use

    Lentiviral

    Tags

    Biotin ligase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pLKO-shAUF1 hairpin 1

    Plasmid

    #52921

    Purpose

    expresses a hairpin specific for human AUF1

    Depositor

    Sheila Stewart

    Article

    Alspach et al Cancer Discov. 2014 Mar 26.

    Insert

    shRNA targeting AUF1 (HNRNPD Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pMKO shRNA Bmf

    Plasmid

    #17236

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bmf shRNA (BMF Human)
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pSLQ1371-sgITGB1-1

    Plasmid

    #121533

    Purpose

    sgITGB1-1 sequence: GAAGCAGGGCCAAATTGTGGG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Ovijit Chaudhuri, Stanley Qi

    Article

    Lee et al Sci Rep. 2019 Nov 20;9(1):17188. doi: 10

    Insert

    sgITGB1-1
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    mouse U6

    Available Since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEBTetD-SNAP-Centrin2

    Plasmid

    #136785

    Purpose

    Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

    Depositor

    Kai Johnsson

    Article

    Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

    Insert

    SNAP-Centrin2 (CETN2 Synthetic, Human)
    

    Tags

    FLAG-tag / His-tag

    Expression

    Mammalian

    Promoter

    CMV-TetO2

    Available Since

    March 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-EF1a-V5-Emerin

    Plasmid

    #120246

    Purpose

    Expresses V5-tagged Emerin in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Emerin (Emd Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXIP TGBRI K to R

    Plasmid

    #19150

    Depositor

    Joan Massague

    Article

    Padua et al Cell. 2008 Apr 4. 133(1):66-77.

    Insert

    Transforming growth factor, beta receptor I (TGFBR2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K420R mutation

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pLenti CMV_mIFP IRES EGFP

    Plasmid

    #72443

    Purpose

    Expressing mIFP and EGFP in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

    Insert

    mIFP T2A HO1 IRES EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pLKO-shAUF1 hairpin 2

    Plasmid

    #52922

    Purpose

    expresses a hairpin specific for human AUF1

    Depositor

    Sheila Stewart

    Article

    Alspach et al Cancer Discov. 2014 Mar 26.

    Insert

    shRNA targeting AUF1 (HNRNPD Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only