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FFiBlast MSCV Puro

Plasmid

#68464

Purpose

Retroviral plasmid for establishment of blasticidin resistance Cre-reporter cell line

Depositor

Tyler Jacks

Publication

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Blasticidin Resistance

Use

Cre/Lox and Retroviral

Expression

Mammalian

Mutation

Inverted

Promoter

LTR

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pVRa32_1122

Plasmid

#49689

Depositor

Christopher Voigt

Publication

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

ECF32_1122

Use

Synthetic Biology

Tags

His6-PreScission

Expression

Bacterial

Mutation

Codon optimized for E.coli

Promoter

pT7-lacO

Available since

Jan. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV10/3xflag-Clock L57E

Plasmid

#47337

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L57E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCMV10/3xflag-Clock L74E

Plasmid

#47338

Purpose

Mutation used for co-IPs

Depositor

Joseph Takahashi

Publication

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

3x Flag

Expression

Mammalian

Mutation

L74E

Promoter

CMV

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pDONR223-ULK2

Plasmid

#23474

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ULK2 (ULK2 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDONR223-FXN

Plasmid

#23620

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FXN (FXN Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-FN3KRP

Plasmid

#23679

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

FN3KRP (FN3KRP Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Publication

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

30nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-15nmEML-mRFP

Plasmid

#231177

Purpose

Stabilizes the ER-mitochondrial distance at 15 nm; tagged with mRFP

Depositor

Dmitry Lim

Publication

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

15nmEML-mRFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET11a-AldoA

Plasmid

#224925

Purpose

Inducible expression of human aldolase A

Depositor

Liskin Swint-Kruse

Publication

Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

Insert

Aldolase A (ALDOA Human)

Expression

Bacterial

Promoter

T7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-JAK3(M511)_IRES_GFP

Plasmid

#206290

Purpose

Expression of mutant JAK3 (M511I) that is able to drive constitutive activation of the JAK/STAT pathway

Depositor

Charles de Bock

Publication

Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

Insert

JAK3 (M511I)

Use

Retroviral

Expression

Mammalian

Mutation

Methionine 511 to Isoleucine

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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THOC1

Plasmid

#156081

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC1 (THOC1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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SYMPK

Plasmid

#156071

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Publication

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SYMPK (SYMPK Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Dcst1-3xFLAG

Plasmid

#183542

Purpose

Expression vector of mouse DC-STAMP domain containing 1 (Dcst1) tagged with 3xFLAG at C-terminus.

Depositor

Masahito Ikawa

Publication

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

DC-STAMP domain containing 1 (Dcst1 Mouse)

Tags

Flag

Expression

Mammalian

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-cofilin S3A

Plasmid

#163604

Purpose

Expresses constitutively active cofilin in mammalian cells

Depositor

Takeshi Imai

Publication

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

cofilin1 (Cfl1 Mouse)

Expression

Mammalian

Mutation

S3A substitution to make cofilin constitutively a…

Available since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)

Plasmid

#136546

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Publication

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Tags

ANXA2 (23-339) and His-tag

Expression

Bacterial

Promoter

T7

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-human Beta-catenin R1C (138–781)

Plasmid

#132608

Purpose

expresses human beta-catenin R1C (138–781) in E. coli

Depositor

Wenqing Xu

Publication

Xing et al Structure. 2008 Mar;16(3):478-87. doi: 1

Insert

Beta-catenin R1C (138–781)

Expression

Bacterial

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSMPUW_6.19_Ex_Ef1a-EGFP-HA-SV40-Zeo

Plasmid

#128455

Purpose

lentiviral expression vector with Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeo Resistance gene

Depositor

Lindy Barrett

Insert

Ef1a promoter, EGFP gene, C-terminal HA tag, SV40 promoter, and Zeocin Resistance gene

Use

Lentiviral

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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LI F-G Spec

Plasmid

#104521

Purpose

LI resistance gene module

Depositor

Thomas Lahaye

Publication

Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

Insert

Spectinomycin resistance gene

Use

Cloning vector

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-Neuroserpin

Plasmid

#58259

Purpose

Retroviral vector for expressing Neuroserpin in mammalian cells

Depositor

Joan Massague

Publication

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only