We narrowed to 13,943 results for: FLU;
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Plasmid#177916PurposeExpresses Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.DepositorInsertTornado-Squash-SAM-sensor 4-2
ExpressionMammalianPromoterU6+27Available SinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-pntAB
Plasmid#158609PurposeLow phosphate induction of pntABDepositorInsertpntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)
ExpressionBacterialPromoterInsulated ugpBp from E. coliAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-C-DiB-RM
Plasmid#168478PurposeC-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168476: pET11a-N-DiB-RM to obtain DiB-RM‐split proteinDepositorInsertC-fragment of DiB-RM
ExpressionBacterialPromoteraraBADAvailable SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-N-DiB-RM
Plasmid#168476PurposeN-fragment of the DiB-RM‐split protein. This plasmid needs to be co-transformed with Plasmid 168478: pMRBad-C-DiB-RM to obtain DiB-RM‐split proteinDepositorInsertN-fragment of DiB-RM
TagsHis-tagExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-N-DiB2
Plasmid#168473PurposeN-fragment of the DiB2‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168256: pMRBad-Z-C-wtBlc to obtain DiB2‐split-Zip proteinDepositorInsertN-fragment of DiB2 + Leucine Zipper
TagsHis-tagExpressionBacterialPromoterT7Available SinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET19b-rDAD2 cPGFP(N180)
Plasmid#166001PurposeExpression of coding sequence in Escherichia coliDepositorInsertrDAD2 cPGFP(N180)
ExpressionBacterialMutationLSSmOrange- V49A (please see depositor comments)PromoterT7 promoterAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-Cas9
Plasmid#120352PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). SpCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromotertet-on and CBhAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-KRAB-dCas9
Plasmid#120355PurposepiggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry). KRAB-dCas9 is constitutively expressed.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonExpressionMammalianPromotertet-on and CBhAvailable SinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2113 - [4-1] - Promoter - Pmlc-1 (w ATG)
Plasmid#159888PurposeThree-fragment Gateway compatible promoter for expression in all muscles of C. elegansDepositorInsert[4-1] - Promoter - Pmlc-1 (w ATG)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM223 - [4-1] - Promoter - Pmlc-2(no_start)
Plasmid#159889PurposeThree-fragment Gateway compatible promoter for expression in all muscles of C. elegansDepositorInsert[4-1] - Promoter - Pmlc-2(no_start)
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD
Plasmid#162811PurposeControl STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.DepositorInsertBsmBI_(C1A)-Intein_DD
UsePiggybacExpressionMammalianMutationIntein(Cys1,Ala)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_M
Plasmid#162815PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_MDepositorInsertUb-(N-TAG)CoV2_M
UsePiggybacExpressionMammalianMutationCoV2_M(Met1,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_9b
Plasmid#162816PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_9bDepositorInsertUb-(N-TAG)CoV2_9b
UsePiggybacExpressionMammalianMutationCoV2_9b(Met1,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF6
Plasmid#162817PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF6DepositorInsertUb-(N-TAG)CoV2_ORF6
UsePiggybacExpressionMammalianMutationCoV2_ORF6(Met1,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF7b
Plasmid#162818PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF7bDepositorInsertUb-(N-TAG)CoV2_ORF7b
UsePiggybacExpressionMammalianMutationCoV2_ORF7b(Met1,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF10
Plasmid#162819PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF10DepositorInsertUb-(N-TAG)CoV2_ORF10
UsePiggybacExpressionMammalianMutationCoV2_ORF10(Met1,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF9c/14
Plasmid#162820PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF9c/14DepositorInsertUb-(N-TAG)CoV2_ORF9c/14
UsePiggybacExpressionMammalianMutationCoV2_ORF9c/14(Met1,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt
Plasmid#162821PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b_altDepositorInsertUb-(N-TAG)CoV2_ORF3b_alt
UsePiggybacExpressionMammalianMutationCoV2_ORF3b_alt(Met1,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b(CoV1h)
Plasmid#162822PurposeSTELLA plasmid for amber suppression of N-terminally labelled CoV2_ORF3b(CoV1h)DepositorInsertUb-(N-TAG)CoV2_ORF3b(CoV1h)
UsePiggybacExpressionMammalianMutationCoV2_ORF3b(CoV1h)(Met1,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD
Plasmid#162805PurposeSTELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integrationDepositorInsertUb-BsmBI-HA_IRES_Blasticidin
UsePiggybacTagsHAExpressionMammalianPromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only