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pDONR223_KRAS_p.G13C

Plasmid

#82809

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G13C

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PTEN_p.R173H

Plasmid

#81682

Purpose

Gateway Donor vector containing PTEN, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PTEN (PTEN Human)

Use

Gateway Cloning

Mutation

R173H

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-N 6bE6

Plasmid

#37915

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 6b E6 (E6 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223_ERBB2_p.T182I

Plasmid

#81705

Purpose

Gateway Donor vector containing ERBB2,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway Cloning

Mutation

T182I

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-N 11E6

Plasmid

#37872

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 11 E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 25, 2012

Availability

Academic Institutions and Nonprofits only
MSCV-C 5E6

Plasmid

#37888

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 5 E6 (E6 HPV)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

July 26, 2012

Availability

Academic Institutions and Nonprofits only
pHAGE-PTEN-R173C

Plasmid

#116632

Purpose

Lentiviral expression of PTEN R173C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PTEN (PTEN Human)

Use

Lentiviral

Mutation

R173C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_MAP2K4_p.S184L

Plasmid

#81681

Purpose

Gateway Donor vector containing MAP2K4, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K4 (MAP2K4 Human)

Use

Gateway Cloning

Mutation

S184L

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-C 8E7

Plasmid

#37920

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 8 E7

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223_KRAS_p.E62K

Plasmid

#81656

Purpose

Gateway Donor vector containing KRAS, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

E62K

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.G12A

Plasmid

#81277

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway Cloning

Mutation

G12A

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-C 8E6

Plasmid

#37919

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 8 E6

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only
pDONR223_GTF2A2_WT_V5

Plasmid

#82997

Purpose

Gateway Donor vector containing GTF2A2, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

GTF2A2 (GTF2A2 Human)

Use

Gateway Cloning

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.T331M

Plasmid

#81450

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway Cloning

Mutation

T331M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.A47V

Plasmid

#81455

Purpose

Gateway Donor vector containing IDH2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway Cloning

Mutation

A47V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IDH2_p.A416V

Plasmid

#81443

Purpose

Gateway Donor vector containing IDH2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IDH2 (IDH2 Human)

Use

Gateway Cloning

Mutation

A416V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_KRAS_p.G12Y

Plasmid

#82799

Purpose

Gateway Donor vector containing KRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, Matthew Meyerson, David Root

Article

Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

Insert

KRAS (KRAS Human)

Use

Gateway Cloning

Mutation

G12Y

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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sgMST1/2-2

Plasmid

#229430

Purpose

knockout of MST1 and MST2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMST1/2-2 (MST1, STK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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sgMST1/2-1

Plasmid

#229429

Purpose

knockout of MST1 and MST2

Depositor

Christopher Vakoc

Article

Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

Insert

sgMST1/2-1 (MST1, STK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6;bU6;EFS

Available Since

Dec. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pInducer-myc-ERK1-R84S/S170D/D338N

Plasmid

#213595

Purpose

Doxycycline inducible expression of myc-tagged ERK1-R84S/S170D/D338N cDNA in mammilian cells

Depositor

Channing Der

Article

Klomp et al Science. 2024 Jun 7;384(6700):eadk0850.

Insert

MAPK3 (MAPK3 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

R84S, S170D, D338N

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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