We narrowed to 59,446 results for: cloning

1. pENTR-R4R3-stop

   Plasmid

   #113745

   Purpose

   Contains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   stop cassette
   

   Use

   CRISPR; Gateway entry vector

   Mutation

   Added 3 stop codons to the original sequence; see…

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNP1 AND input A

   Plasmid

   #107362

   Purpose

   Input A for pNP1 AND gate sfGFP

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   AND gate input A
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNP1 AND input B

   Plasmid

   #107363

   Purpose

   Input B for pNP1 AND gate sfGFP

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   AND gate input B
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28b-NmCas9-Flag H588A

   Plasmid

   #109032

   Purpose

   H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

   Depositor

   Yan Zhang

   Article

   Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

   Insert

   CRISPR-associated endonuclease Cas9
   

   Tags

   Flag Tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDIRECT_37J

   Plasmid

   #91153

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:TALEN, Plant Selection: nos:hpt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:TALEN
   

   Use

   TALEN

   Expression

   Plant

   Available Since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCVD447

   Plasmid

   #78569

   Purpose

   E. coli E2348/69 LEE region (Type 3 Secretion System) cloned into pBluescriptKS.

   Depositor

   James Kaper

   Article

   Jarvis et al Proc Natl Acad Sci U S A. 1995 Aug 15;92

   Insert

   lee operon
   

   Use

   Cloning vector

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pQTEV-NEUGRIN

   Plasmid

   #34804

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

   Insert

   NEUGRIN (NGRN Human)
   

   Tags

   His and TEV

   Expression

   Bacterial

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFOSGE_COF3E07

   Plasmid

   #70987

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   TfIIS (TfIIS Fly)
   

   Use

   Gateway entry vector

   Available Since

   Dec. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pFOSGE_COF3B09

   Plasmid

   #70998

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Hrb87F (Hrb87F Fly)
   

   Use

   Gateway entry vector

   Available Since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pQTEV-C10orf83

    Plasmid

    #34827

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    C10orf83 (MORN4 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSYL017

    Plasmid

    #246593

    Purpose

    template plasmid for site-saturation mutagenesis library cloning

    Depositor

    Tina Wang

    Article

    Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

    Insert

    AtRaf1 variant 2g (AT3G04550 Mustard Weed)
    

    Expression

    Bacterial

    Mutation

    AtRaf1L121F/I127L/A195V/N351Y/R432G

    Available Since

    Oct. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PB-TRE3G-CCRB

    Plasmid

    #236772

    Purpose

    A piggybac-based cloning vector containing TRE3G promoter followed by cloning site and CAG promoter-driven nuclear-localized Clover-P2A-rtTA-IRES-BSD.

    Depositor

    Kazutoshi Takahashi

    Article

    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do

    Type

    Empty backbone

    Use

    Piggybac

    Expression

    Mammalian

    Promoter

    TRE3G promoter, CAG promoter

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MCUb-HA-pLYS5

    Plasmid

    #236790

    Purpose

    Clone MCUB into pLYS5 (Hygro resistance) with HA tag

    Depositor

    Dipayan Chaudhuri

    Article

    Rai et al J Cell Physiol. 2025 Apr;240(4):e70033.

    Insert

    MCUB (MCUB Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pXTK-BB6 [Backbone_RK2-KanR-TetR-PaqCI-Compatible_PartTypeBB]

    Plasmid

    #229392

    Purpose

    XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR-TetR-PaqCI-Compatible_PartTypeBB and an sfGFP dropout

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXTK-BB7 [Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB]

    Plasmid

    #229393

    Purpose

    XanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_repABC-pMlb-KanR-TetR-PaqCI-Compatible_PartTypeBB and an sfGFP dropout

    Depositor

    Michael Springer

    Article

    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FUGW-RUSH-Strep-KDEL

    Plasmid

    #228527

    Purpose

    Lentiviral vector, cloning vector for Strep-KDEL-IRES in mammalian cells.

    Depositor

    Ginny Farias

    Article

    Li et al Nat Commun. 2024 Dec;15: 10829. doi: 10.

    Insert

    Strep-KDEL
    

    Use

    Lentiviral

    Tags

    Streptavidin-KDEL

    Expression

    Mammalian

    Promoter

    Ubc

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. JDW 1051 (pME_V5_GATA2_P2A_H2A_mCherry)

    Plasmid

    #232128

    Purpose

    A middle entry gateway clone containing V5 tagged human GATA2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    GATA2 (GATA2 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. JDW 1034 (pME_V5_TCF7_P2A_H2A_mCherry)

    Plasmid

    #232124

    Purpose

    A middle entry gateway clone containing V5 tagged human TCF7 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    TCF7 (TCF7 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. JDW 1031 (pME_V5_LEF1_P2A_H2A_mCherry)

    Plasmid

    #232123

    Purpose

    A middle entry gateway clone containing V5 tagged human LEF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    LEF1 (LEF1 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. JDW 1060 (pME_V5_FOXF2_P2A_H2A_mCherry)

    Plasmid

    #232131

    Purpose

    A middle entry gateway clone containing V5 tagged human FOXF2 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    FOXF2 (FOXF2 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge