We narrowed to 121,130 results for: cloning

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pCSDest2

Plasmid

#22424

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Type

Empty backbone

Use

Gateway Cloning

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCSDest2

Plasmid

#22424

Depositor

Nathan Lawson

Publication

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Type

Empty backbone

Use

Gateway Cloning

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pEF-ENTR-LacZ (710-1)

Plasmid

#17430

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

LacZ

Use

Gateway Cloning

Tags

His and V5

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENTR4-GST 6P-1 (w487-1)

Plasmid

#17741

Purpose

Gateway entry vector for N-term GST fusion, frame 6P-1

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

GST

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pENTR4-myc-nuc (w556-1)

Plasmid

#20026

Purpose

Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

Myc and NLS

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pEF-ENTR-LacZ (710-1)

Plasmid

#17430

Purpose

Gateway entry vector with EF1-a promoter, V5-His C-term, Lac Z

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

LacZ

Use

Gateway Cloning

Tags

His and V5

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENTR4-GST 6P-1 (w487-1)

Plasmid

#17741

Purpose

Gateway entry vector for N-term GST fusion, frame 6P-1

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

GST

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pENTR4-myc-nuc (w556-1)

Plasmid

#20026

Purpose

Gateway entry vector for nuclear targeting of domains (C-term NLS, myc tags)

Depositor

Eric Campeau, Paul Kaufman

Publication

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning

Tags

Myc and NLS

Expression

Mammalian

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pDEST17-UbE2B

Plasmid

#15781

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2B (UBE2B Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pDEST17-UbE2B

Plasmid

#15781

Depositor

Wade Harper

Publication

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Ubiquitin conjugating enzyme UbE2B (UBE2B Human)

Tags

His

Expression

Bacterial

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3 V5 hHR23A

Plasmid

#13053

Depositor

Steven Grossman

Publication

Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hHR23A (RAD23A Human)

Tags

His and V5

Expression

Mammalian

Mutation

R325K and L355P relative to NP_001257291.1

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 V5 hHR23A

Plasmid

#13053

Depositor

Steven Grossman

Publication

Brignone et al Oncogene. 2004 May 20. 23(23):4121-9.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hHR23A (RAD23A Human)

Tags

His and V5

Expression

Mammalian

Mutation

R325K and L355P relative to NP_001257291.1

Available since

Oct. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCH45 (multiAsCas12a-KRAB lenti)

Plasmid

#217330

Purpose

Lentiviral expression of multiAsCas12a-KRAB

Depositor

Luke Gilbert

Publication

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

R1226A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCH45 (multiAsCas12a-KRAB lenti)

Plasmid

#217330

Purpose

Lentiviral expression of multiAsCas12a-KRAB

Depositor

Luke Gilbert

Publication

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Insert

multiAsCas12a

Use

CRISPR and Lentiviral

Tags

6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2

Mutation

R1226A/E174R/S542R/K548R

Promoter

UCOE-SFFV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET-MCN-10His-mStayGold (E138D)

Plasmid

#211361

Purpose

Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Publication

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Tags

10xHis-tag

Expression

Bacterial

Mutation

E138D

Promoter

T7

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET-MCN-10His-mStayGold (E138D)

Plasmid

#211361

Purpose

Bacterial expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Codon optimised for expression in Escherichia coli. Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Publication

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Tags

10xHis-tag

Expression

Bacterial

Mutation

E138D

Promoter

T7

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Publication

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV_IRES_GFP_CD16a

Plasmid

#196189

Purpose

Retroviral expression plasmid for generating stable FCGR3A (CD16a)-expressing cell lines.

Depositor

Ana Ruiz-Saenz

Publication

Vincken et al STAR Protoc. 2023 Apr 17;4(2):102224. do

Insert

CD16a (FCGR3A Human)

Use

Retroviral

Expression

Mammalian

Mutation

changed Phenylalanine 158 to Valine

Promoter

LTR

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJR101

Plasmid

#187241

Purpose

Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for Perturb-seq with mU6 promoter

Use

Lentiviral

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJR101

Plasmid

#187241

Purpose

Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Publication

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for Perturb-seq with mU6 promoter

Use

Lentiviral

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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