163,765 results

1. TFORF2165

   Plasmid

   #141953

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   GRHL3 (GRHL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

   Plasmid

   #87306

   Purpose

   An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

   Depositor

   Li Zhang

   Article

   Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre-dependent mouse codon-optimized Flp recombinase.
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXPR_502_sgCD45

   Plasmid

   #158705

   Purpose

   CD45 CRISPRa control

   Depositor

   David Root, John Doench

   Article

   Orchard et al J Virol. 2018 Dec 10;93(1). pii: JVI.013

   Insert

   CD45 (PTPRC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. VN-Tau (P301L)

   Plasmid

   #87634

   Purpose

   expresses hTau fused to the N-terminal part of Venus

   Depositor

   Tiago Outeiro

   Insert

   MAPT (MAPT Human)
   

   Use

   Tags

   N-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Mutation P301L

   Promoter

   CMV

   Available since

   Oct. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GPR120-Tango

   Plasmid

   #66310

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR120 (FFAR4 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-SEP-GluR1

   Plasmid

   #24000

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   GluR1 (Gria1 Rat)
   

   Use

   Tags

   GluR1 signal peptide (aa1-22) and SEP (supereclip…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLJC6-2XFLAG-TMEM192

   Plasmid

   #104435

   Purpose

   Expresses 2XFLAG-TMEM192 in mammalian cells

   Depositor

   David Sabatini

   Article

   Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

   Insert

   TMEM-192 (TMEM192 Human)
   

   Use

   Lentiviral

   Tags

   2XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CAG-mTagBFP2-P2A-EGFP

   Plasmid

   #184943

   Purpose

   Expresses blue fluorescent protein mTagBFP2 and EGFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   mTagBFP2-P2A-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ10844

   Plasmid

   #183956

   Purpose

   CAG-hyperdCas12a-HA-miniVPR-mCherry-T2A-HygroR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   HyperdCas12a
   

   Use

   CRISPR

   Tags

   HA, Hygromycin Resistance, and mCherry

   Expression

   Mammalian

   Mutation

   D832A, D156R, E292R, D235R, D350R

   Promoter

   CAG

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV4-3 HA/IkB-alpha

    Plasmid

    #21985

    Depositor

    Warner Greene

    Article

    Sun et al Mol Cell Biol. 1996 Mar . 16(3):1058-65.

    Insert

    I-kappa B alpha (NFKBIA Human)
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. tetO-SMAD6

    Plasmid

    #170700

    Purpose

    doxycycline-inducible overexpression of SMAD6

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    SMAD6 (SMAD6 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEx-4T-1-YTHDF2

    Plasmid

    #52301

    Purpose

    Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pCAG-mGFP

    Plasmid

    #14757

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Has Service

    Cloning Grade DNA

    Insert

    membrane bound form of EGFP
    

    Use

    Tags

    Palmitoylation signal

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSCALPS-FLAG-HA-mmuMettl3

    Plasmid

    #207122

    Purpose

    Lentiviral plasmid to express mouse Mettl3

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    Mettl3 (Mettl3 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mutation

    Promoter

    SSFV

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-INSR-V5/HIS

    Plasmid

    #201980

    Purpose

    expression of human INSR receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    INSR receptor tyrosine kinase (INSR Human)
    

    Use

    Tags

    V5, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti SYN-dCas9-KRAB-MeCP2

    Plasmid

    #155365

    Purpose

    Expresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoter

    Depositor

    Jeremy Day

    Article

    Duke et al Front Genome Ed. 2020 Sep 15;2:9. doi: 1

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    3x FLAG and 7x His

    Expression

    Mammalian

    Mutation

    Promoter

    SYN

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK5-myc-Rac1-Q61L

    Plasmid

    #12983

    Depositor

    Gary Bokoch

    Insert

    Rac1 constitutively active (RAC1 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Q61L Constitutively active

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TO-hNIL

    Plasmid

    #172113

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Article

    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

    Inserts

    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Tags

    T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a and TRE3G

    Available since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQP-T2A

    Plasmid

    #102583

    Purpose

    Light-controlled activation of expression

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

    Insert

    BphP1-VP16-T2A-NLS-GAL4-QPAS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCX-SponGee

    Plasmid

    #134775

    Purpose

    mammalian expression of SponGee, a buffer of cGMP that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

    Insert

    SponGee
    

    Use

    Tags

    FLAG and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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