167,318 results

1. pLexM-SLC3A2

   Plasmid

   #197994

   Purpose

   Expresses SLC3A2 as an N-terminal His tagged construct in mammalian cells

   Depositor

   Simon Newstead

   Article

   Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

   Insert

   SLC3A2 (SLC3A2 Human)
   

   Tags

   His tag

   Expression

   Mammalian

   Mutation

   Please see depositor comments below

   Promoter

   CMV

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST40-Arl13b11

   Plasmid

   #40873

   Depositor

   Tamara Caspary

   Article

   Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

   Insert

   Arl13b (Arl13b Mouse)
   

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   no stop codon

   Promoter

   CMV

   Available Since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pFUW-tetO-Batf3

   Plasmid

   #139837

   Purpose

   doxycycline-inducible expression of mouse Batf3 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

   Insert

   Batf3 (Batf3 Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE-mCMV

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. HER2 CA (V659E)

   Plasmid

   #16259

   Depositor

   Mien-Chie Hung

   Article

   Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

   Insert

   HER2 (ERBB2 Human)
   

   Expression

   Mammalian

   Mutation

   Constitutively active: V659E

   Available Since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO5.sgRNA.EFS.GFP

   Plasmid

   #57822

   Purpose

   3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   sgRNA
   EFS
   eGFP
   

   Use

   CRISPR and Lentiviral

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR.CMV

   Plasmid

   #32688

   Purpose

   Mammalian expression and gateway entry vector

   Depositor

   Ming-Sound Tsao

   Article

   Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNR220

   Plasmid

   #79541

   Purpose

   Input plasmid: expresses three serine recombinases (TP901, BxbI, and A118) downstream of three inducible promoters.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Inserts

   TP901
   BxbI
   A118
   

   Tags

   His tag and LAA degradation tag

   Expression

   Bacterial

   Available Since

   July 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.3_SOX2

   Plasmid

   #26817

   Depositor

   Derrick Rossi

   Article

   Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

   Insert

   5'UTR-SOX2-3'UTR (SOX2 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 17, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pBabe_puro_DEST_Flag_SKIL

   Plasmid

   #45265

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   SNON (SKIL Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pT7-7 asyn WT

    Plasmid

    #36046

    Depositor

    Hilal Lashuel

    Article

    Paleologou et al J Biol Chem. 2008 Jun 13;283(24):16895-9

    Insert

    alpha-synuclein (SNCA Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAdx-CMV-copGFP

    Plasmid

    #73346

    Purpose

    Express copGFP under CMV promoter

    Depositor

    Kazuhiro Oka

    Insert

    CMV-copGFP
    

    Use

    Adenoviral

    Promoter

    CMV

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. EF1a_mCherry_P2A_Hygro_Barcode

    Plasmid

    #120426

    Purpose

    Barcoded lentiviral vector to express mCherry in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    mCherry
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3 2 kb prom. CD274

    Plasmid

    #107003

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. GPR171-DuET

    Plasmid

    #213269

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR171 (GPR171 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. RFP Dynamin2 K44A

    Plasmid

    #128153

    Purpose

    Expression of Dynamin 2 K44A

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Chua et al Proc Natl Acad Sci U S A. 2009 Dec 8;106

    Insert

    Dynamin 2 (Dnm2 Rat)
    

    Expression

    Mammalian

    Mutation

    K44A

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK425 (GAL-OsTIR1(F74A))

    Plasmid

    #140657

    Purpose

    GAL-OsTIR1(F74A)

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    GAL-OsTIR1(F74A)
    

    Expression

    Yeast

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX307 GFP-Kif26b-C

    Plasmid

    #118483

    Purpose

    lentiviral expression of GFP-Kif26b-C in mammalian cells

    Depositor

    Henry Ho

    Article

    Karuna et al Genes (Basel). 2018 Apr 5;9(4). pii: gen

    Insert

    mouse Kif26b-C (Kif26b Mouse)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Encodes GFP fused to amino acid 1735 to 2112 of m…

    Promoter

    EF1A

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. p667-UBC-NEK7-V5-miniTurbo_IDG-K

    Plasmid

    #189882

    Purpose

    Gateway destination clone of NEK7 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    NEK7 (NEK7 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5-miniTurbo

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330S-2

    Plasmid

    #58778

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1/Puro-CAG-JEDI-2P

    Plasmid

    #179458

    Purpose

    Genetically encoded voltage indicator (GEVI) JEDI-2P expressed under strong mammalian promoter (CAG)

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Insert

    JEDI-2P
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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