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pEY14

Plasmid

#191059

Purpose

acr-5 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-5(orf) (acr-5 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-dInPAkt(R23/25A)

Plasmid

#181835

Purpose

Phospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-dInPAkt

Tags

N-terminal targeting sequence from Lyn kinase, dd…

Expression

Mammalian

Mutation

Arginines 23 and 25 in PH domain mutated to alani…

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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EKARdual

Plasmid

#99870

Purpose

Measure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrate

Depositor

Marc Tramier

Article

Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

Insert

EKAR

Tags

ShadowG and mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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-2.5Kb MMP10

Plasmid

#53973

Purpose

Luciferase reporter for mouse MMP10 2.5kb promoter

Depositor

Michael Chin

Article

Wu et al Biochem Biophys Res Commun. 2011 Dec 2;4

Insert

mouse MMP10 2.5Kb promoter (Mmp10 Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PCR from C57BL6/J

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-ABE5

Plasmid

#195371

Purpose

dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x8

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

ecTadA8e-(GGGGS)x8-dLbCas12a-D156R

Use

CRISPR; Gateway compatible ectada8e-(ggggs)x8-dlb…

Tags

5' and 3' NLS

Expression

Plant

Promoter

ZmUBI1

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_hH1

Plasmid

#102841

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_hH1

Use

Pcr template for stagr inserts

Promoter

hH1

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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TB:6xAP-1_RE:MiniP

Plasmid

#133884

Purpose

6 copies of the AP-1 binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

6 copies of the AP-1 binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE651

Plasmid

#153229

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI

Plasmid

#115216

Purpose

Expresses Pin2Cas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

Pin2Cas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Ago1

Plasmid

#21534

Depositor

Edward Chan

Article

Lian et al RNA. 2009 May . 15(5):804-13.

Insert

Ago1 (AGO1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 13, 2009

Availability

Academic Institutions and Nonprofits only
pemiRFP703-tubulin

Plasmid

#136564

Purpose

Human alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

emiRFP703-tubulin

Tags

emiRFP703

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-NRCH-PE2-BSD

Plasmid

#191101

Purpose

Lentiviral expression plasmid of NRCH-PE prime editor with P2A-BSD marker

Depositor

Hyongbum Kim

Article

Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

Insert

NRCH-PE2-BSD

Use

CRISPR and Lentiviral

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF-1a

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI

Plasmid

#115208

Purpose

Expresses PinCas13b from a SUMO-tagged bacterial expression construct

Depositor

Feng Zhang

Article

Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

Insert

PinCas13

Tags

SUMO

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR221-NR1H3

Plasmid

#79514

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H3 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

LXR Alpha (NR1H3 Human)

Use

Multisite gateway entry clone

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJ3 SHP2 WT

Plasmid

#8317

Depositor

Ben Neel

Article

Bennett et al Mol Cell Biol 1996 Mar;16(3):1189-202.

Insert

SHP2 (PTPN11 Human)

Expression

Mammalian

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only

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pDGE342

Plasmid

#153251

Purpose

M8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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7xSMAD_RE::GrRenilla

Plasmid

#178319

Purpose

SMAD GreenRenilla luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

Insert

SMAD GreenRenilla Reporter

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-tTA

Plasmid

#193338

Purpose

AAV-mediated expression of tTA under the CAG promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tTA2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDGE497

Plasmid

#153249

Purpose

M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMXS-IP Keima-Pex11B

Plasmid

#224558

Purpose

expresses Keima fused to PEX11B in mammalian cells

Depositor

William Kaelin, Gregory Wyant

Article

Wyant et al Circulation. 2024 Jun 17. doi: 10.1161/C

Insert

PEX11B (PEX11B Human)

Use

Retroviral

Tags

Keima

Expression

Mammalian

Available Since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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