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pNT-3

Plasmid

#154934

Purpose

3 designed non-target sites

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

3 designed non-target sites

Expression

Bacterial

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pNT-1

Plasmid

#154935

Purpose

1 designed non-target site

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

1 designed non-target sites

Expression

Bacterial

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pJZC530

Plasmid

#135434

Purpose

Yeast integrating plasmid for pTETO1-Venus reporter gene

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

pTETO1_Venus

Expression

Yeast

Promoter

pTETO1

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJS032

Plasmid

#135431

Purpose

Yeast integrating plasmid to express PYL1-VP64

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

PYL1-VP64

Expression

Yeast

Promoter

pAdh

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJS033

Plasmid

#135432

Purpose

Yeast integrating plasmid to express MCP-ABI

Depositor

Jesse Zalatan

Article

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

MCP-ABI

Expression

Yeast

Promoter

pTEF1

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pNX3a-PK9

Plasmid

#78356

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

9xPK
kanMX6

Expression

Yeast

Available Since

March 10, 2017

Availability

Academic Institutions and Nonprofits only

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pNXRVg-HACre

Plasmid

#78405

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
arg3+ (arg3 Fission Yeast)

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNXRVh-HACre

Plasmid

#78406

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
leu1+ (leu1 Fission Yeast)

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNXRVk-HACre

Plasmid

#78408

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
ade6+ (ade6 Fission Yeast)

Expression

Yeast

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3d-PK3

Plasmid

#78375

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xPK
ura4+ (ura4 Fission Yeast)

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3d-PK6

Plasmid

#78376

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

6xPK
ura4+ (ura4 Fission Yeast)

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3d-PK9

Plasmid

#78377

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

9xPK
ura4+ (ura4 Fission Yeast)

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3d-PK12

Plasmid

#78378

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

12xPK
ura4+ (ura4 Fission Yeast)

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3a-HA3

Plasmid

#78359

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
kanMX6

Available Since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pLG173

Plasmid

#124368

Purpose

Control plasmid with only spectinomycin

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

empty backbone (control) (NEWENTRY Synthetic)

Expression

Bacterial

Promoter

No promoter

Availability

Academic Institutions and Nonprofits only

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pLG320

Plasmid

#124370

Purpose

Vanillic acid inducible system for expression in B. subtilis

Depositor

Christopher Voigt

Article

Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

Insert

gfpmut2

Expression

Bacterial

Promoter

Pspank(V)

Availability

Academic Institutions and Nonprofits only

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pAKW.031-GM-R5

Plasmid

#154044

Purpose

Expresses R5 peptide in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Silaffin R5 peptide

Tags

Maltose binding protein and his tag

Expression

Bacterial

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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E. coli MEV20

Bacterial Strain

#197113

Purpose

E. coli BL21(DE3) derivative for diterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
E. coli MEV15

Bacterial Strain

#197112

Purpose

E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
pDY1622 NOLC1 sgRNA 1

Plasmid

#234833

Purpose

sgRNA 1 for NOLC1 STITCHR insertion

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

Insert

NOLC1 sgRNA (NOLC1 Human)

Use

CRISPR

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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