We narrowed to 8,359 results for: 11
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Plasmid#217709PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertADGRL4 (ADGRL4 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-406Available sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA2 CTFdeltaTA-FLAG
Plasmid#217712PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertADGRA2 (ADGRA2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-752Available sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRA1 CTFdeltaTA-FLAG
Plasmid#217711PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertADGRA1 (ADGRA1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-6Available sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG6 CTFdeltaTA-FLAG
Plasmid#217737PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGR6 (ADGRG6 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-846Available sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE1 CTFdeltaTA-FLAG
Plasmid#217722PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertADGRE1 (EMR1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-590Available sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE2 CTFdeltaTA-FLAG
Plasmid#217723PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE2 (ADGRE2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-523Available sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis -MH)
Plasmid#195613PurposeExpresses the wild-type I-SceI homing endonuclease from the T7 promoterDepositorInsertI-SceI
ExpressionBacterialMutationNonePromoterT7Available sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
binder/tag-WT-FAK-insertionB
Plasmid#190440PurposeThe tag is SsrA (AANDENY). Tag is inserted after D405 of FAK.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFAK (Ptk2 Mouse)
TagsmCherryExpressionMammalianMutationSsrA inserted after aa 405Available sinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZCAN
Plasmid#129115PurposeZ. rouxii centromeric vector containing ClonNAT resistanceDepositorTypeEmpty backboneExpressionYeastAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcryBFP_Umyo18bGFP
Plasmid#172527PurposealphaA-crystallin promoter (cry) drives GFP expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish Myo18b.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmyo18b
ExpressionMammalianAvailable sinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
(517) SB KRAB-SadCas9 U6-gStop
Plasmid#163023PurposeSleeping Beauty TET-On expression of CRISPRi with gSTOP gRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMammalian codon-optimized SadCas9
UseTransposonTagsKRAB and myc NLSExpressionMammalianPromoterTet-OnAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Rab10-FLAG
Plasmid#135559PurposeExpress mouse Rab10 in mammalian cells, FLAG at N terminalDepositorAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pECE beta1 R760A
Plasmid#50511DepositorAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3 (T1 truncation)
Plasmid#49210Purposeexpresses Flag tagged mouse Tim3 (T1 truncation)DepositorInsertTim3 T1 truncation (Havcr2 Mouse)
TagsFLAG and signal sequenceExpressionMammalianMutationT1 truncation ( contains all but the three most C…PromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
Flag‐mTim3 (T2 truncation)
Plasmid#49211Purposeexpresses Flag tagged mouse Tim3 (T2 truncation)DepositorInsertTim3 T2 truncation (Havcr2 Mouse)
TagsFLAG and signal sequenceExpressionMammalianMutationT2 truncation ( lacks all but one tyrosine, which…PromoterEF1aAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Tbx20
Plasmid#72697PurposeDoxycyline regulated expression of Tbx20 in mammalian cellsDepositorAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-Syn-DIO-TRPV1-P2A-mCherry
Plasmid#200831PurposeIn the presence of Cre, drives neuron-specific expression of TRPV1 with fluorescence reporter mCherryDepositorAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTSara-C
Plasmid#60722PurposeExpresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORFDepositorInsertResidues 180-880 of T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationOnly amino acids 180-880 of T7 RNAPPromoterPBADAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-nAChr-beta3-YFP
Plasmid#50484Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnAChRBeta3 subunit, isoform 1 (Chrnb3 Mouse)
TagsYFPExpressionMammalianMutationYFP fusion in M3-M4 loop (after residue P379). O…PromoterCMVAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-nAChr-Alpha6-mCherry
Plasmid#50480Purposethis report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRFDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertnAChr-alpha6 (Chrna6 Mouse)
TagsmCherry fusion in M3-M4 loop (after residue A405)ExpressionMammalianPromoterCMVAvailable sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only