Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 14,273 results for: cas9

Clear keywords
Showing: 6921 - 6940 of 14273 results

  3. plenti-Lb-Cas12a-2xNLS

    Plasmid
    #155046
    Purpose
    Lentiviral vector expressing human codon-optimized _C-terminal Myc-tagged Lachnospiraceae bacterium (Lb)-Cas12a nuclease with N- and C-terminal NLS and Neomycin/G418/Geneticin resistance from EF-1alpha promoter
    Depositor
    Benjamin Blencowe, Jason Moffat
    Article
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    LbCas12a
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS, Myc-tag and SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1a
    Available Since
    Aug. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    155046-plasmid-map-sequence-id-308670

    Enlarge

  4. pCSD_2xNES-FRB-ZFPTS2-3xFLAG-2xNLS

    Plasmid
    #107303
    Purpose
    Expresses ZFP-VEGFA-TS2 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    ZFP_VEGFA-TS2
    Use
    CRISPR
    Tags
    2xNES, 3x Flag, 2xNLS, and FRB
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107303-plasmid-map-sequence-id-204639

    Enlarge

  5. pCSD_2xNES-FRB-ZFPTS3-3xFLAG-2xNLS

    Plasmid
    #107304
    Purpose
    Expresses ZFP-VEGFA-TS3 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    ZFP_VEGFA-TS3
    Use
    CRISPR
    Tags
    2xNES, 3x Flag, 2xNLS, and FRB
    Expression
    Mammalian
    Promoter
    CMV IE94
    Available Since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107304-plasmid-map-sequence-id-204643

    Enlarge

  7. pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R

    Plasmid
    #228729
    Purpose
    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Article
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Insert
    Domain inlaid Nme2Smu nCas9 (D16A)-i1-L10-E932R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A E932R
    Promoter
    CMV
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228729-plasmid-map-sequence-id-454007

    Enlarge

  8. pMLS791

    Plasmid
    #188889
    Purpose
    Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
    Depositor
    Erik Jorgensen
    Article
    Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.
    Insert
    floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
    Expression
    Worm
    Mutation
    C. elegans codom optimization and intron addition
    Available Since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    188889-plasmid-map-sequence-id-370713

    Enlarge

  19. pATF4.1.0-gDNA

    Plasmid
    #113772
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4
    Depositor
    Kevin White
    Insert
    ATF4 (ATF4 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113772-plasmid-map-sequence-id-230558

    Enlarge

  20. pKb-TnpB1

    Plasmid
    #215333
    Purpose
    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site
    Depositor
    Kutubuddin Molla
    Article
    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10
    Insert
    Rice codon optimized ISDra2 TnpB
    Tags
    Not applicable
    Expression
    Plant
    Promoter
    Rice ubiquitin promoter for TnpB and OsU3 promoteā€¦
    Available Since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    215333-plasmid-map-sequence-id-446611

    Enlarge

Showing: 6921 - 6940 of 14273 results