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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,947 results for: emb

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Showing: 6921 - 6940 of 11947 results

  1. pBV163

    Plasmid
    #108364
    Purpose
    Used to replace C. glabrata YAP1 promoter with the stronger TDH3 promoter.
    Depositor
    Scott Moye-Rowley
    Publication
    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0
    Insert
    Knock-in Cg TDH3 promoter in Cg YAP1 gene
    Use
    Replace c.glabrata yap1 promoter with c. glabrata…
    Promoter
    TDH3
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108364-plasmid-map-sequence-id-209913

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  2. pUC19-G2ZYP2

    Plasmid
    #103110
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103110-plasmid-map-sequence-id-201819

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  3. pUC19-B8I085

    Plasmid
    #103089
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Publication
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    B8I085(Cas9 coding gene from Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10))
    Use
    CRISPR; Cloning vector
    Mutation
    human codon-optimized
    Available since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103089-plasmid-map-sequence-id-201739

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  5. pPD3C

    Plasmid
    #86971
    Purpose
    Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Publication
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 fused to phosphite dehydrogenase and P450cam
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R in PCNA3, silent mutat…
    Promoter
    T7 promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86971-plasmid-map-sequence-id-173928

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  15. C20-His-bio

    Plasmid
    #52327
    Purpose
    Expresses full-length Adipocyte plasma membrane-associated protein ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    C20 (APMAP Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    52327-plasmid-map-sequence-id-83058

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  16. TXNDC-bio-His

    Plasmid
    #51858
    Purpose
    Expresses full-length Thioredoxin-related transmembrane protein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TXNDC (TMX1 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    51858-plasmid-map-sequence-id-79746

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  18. pChlr-2SR

    Plasmid
    #52146
    Purpose
    Encodes module 2 with amino acids 12S-16R for recognition of nt 7C
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 61-96 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16R in Module 2
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. pChlr-3NQ

    Plasmid
    #52149
    Purpose
    Encodes module 3 with amino acids 12N-16Q for recognition of nt 6U
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 97-132 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12N in Module 3
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  20. pChlr-4CQ

    Plasmid
    #52151
    Purpose
    Encodes module 4 with amino acids 12C-16Q for recognition of nt 5A
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 133-168 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12C in Module 4
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 6921 - 6940 of 11947 results