We narrowed to 87,573 results for: cloning

1. p663-UBC-TurboID-V5-CDK13_IDG-K

   Plasmid

   #135267

   Purpose

   Gateway destination clone of CDK13 (human) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   TurboID-V5-CDK13 (CDK13 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMT-flp

   Plasmid

   #67274

   Purpose

   Conjugally transferable Flp recombinase plasmid for unmarked mutagenesis

   Depositor

   Mark Liles

   Article

   Hossain et al Biotechnol Rep (Amst). 2015 Aug 28;8:24-

   Insert

   tetA, flp, repA101, oriR101
   

   Use

   Flp recombinase plasmid, temperature sensitive

   Tags

   His-SUMO (flp)

   Promoter

   tetA promoter of plasmid pACYC184 origin

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6_(Gluc)_INT(GenPurpClon)

   Plasmid

   #68433

   Purpose

   General Purpose cloning vector for "INT"-like constructs under U6-promoter expression.

   Depositor

   John Rinn

   Article

   Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

   Insert

   INT construct
   

   Use

   CRISPR; Cloning vector for generating int-like co…

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAP-BAC

   Plasmid

   #120229

   Purpose

   TAR cloning in yeast and low-copy maintenance of large constructs in E. coli

   Depositor

   Bradley Moore

   Article

   Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-FLIP-puro-dsRed-GFP-miRNA

   Plasmid

   #32704

   Depositor

   Hans Clevers

   Article

   Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

   Insert

   emGFP-miRNA
   

   Use

   Cre/Lox, RNAi, and Retroviral

   Expression

   Mammalian

   Available Since

   Dec. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDHt-SK

   Plasmid

   #63615

   Purpose

   The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

   Depositor

   Seogchan Kang

   Article

   Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

   Type

   Empty backbone

   Use

   Fungal expression

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL4.23-MYC

   Plasmid

   #86461

   Purpose

   A modified version of the Promega pGL4.23 vector. Contains the MYC promoter in place of the minimal promoter and polyadenylation signals flanking the enhancer cloning site.

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Type

   Empty backbone

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   MYC

   Available Since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. p221z-CAS9p-TagRFP-t35s

   Plasmid

   #118386

   Purpose

   Entry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

   Insert

   CAS9p-TagRFP
   

   Use

   CRISPR

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL4.23-MYC-e3

   Plasmid

   #120341

   Purpose

   pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Insert

   chr8:129056967-129058438 (hg19)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   MYC

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL4.23-MYC-e5

    Plasmid

    #120343

    Purpose

    pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    chr8:130594100-130596043 (hg19)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    MYC

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL4.23-MYC-e4

    Plasmid

    #120342

    Purpose

    pGL4.23-MYC with an enhancer cloned upstream of the MYC promoter.

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    chr8:129060477-129062593 (hg19)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    MYC

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-Gal80ts

    Plasmid

    #51619

    Purpose

    vertebrate expression of full-length temperature-sensitive Gal80 with stop

    Depositor

    Joshua Bonkowsky

    Article

    Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

    Insert

    Gal80ts (GAL80 Budding Yeast)
    

    Use

    Vertebrate expression

    Mutation

    temperature sensitive (G203V, G323R)*

    Available Since

    March 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO GFP HSPA6

    Plasmid

    #19486

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    heat shock 70kDa protein 6 (HSPA6 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDD284

    Plasmid

    #66825

    Purpose

    TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    TagRFP-T-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized TagRFP-T

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3.1/Myc-His(-)-MSulf-2

    Plasmid

    #13008

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    mouse sulfatase 2 (Sulf2 Mouse)
    

    Tags

    6xHis and Myc

    Expression

    Mammalian

    Available Since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKO (Kan)

    Plasmid

    #110086

    Purpose

    Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pG-HIV-2LTR

    Plasmid

    #104590

    Purpose

    standard for qPCR

    Depositor

    Kristine Yoder

    Article

    Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

    Insert

    2LTR circle junction of HIV-1
    

    Use

    Cloning vector

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD283

    Plasmid

    #66824

    Purpose

    YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    YPET-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized YPET

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. KRASB

    Plasmid

    #25153

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    KRASb:M1-K169 (KRAS Human)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Q61H

    Available Since

    Aug. 6, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. CMV-HA-hTAF6

    Plasmid

    #17988

    Depositor

    Naoko Tanese

    Article

    Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

    Insert

    TAF6 (TAF6 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Aug. 19, 2008

    Availability

    Academic Institutions and Nonprofits only