We narrowed to 17,079 results for: Por

1. pAAV-hsyn-GRAB_Ado1.0med

   Plasmid

   #153286

   Purpose

   Express the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons

   Depositor

   Yulong Li

   Article

   Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

   Insert

   Adenosine sensor GRAB_Ado1.0med (medium affinity)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   July 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHR_PGK_LaG17-mCherry-HOTag3

   Plasmid

   #190024

   Purpose

   Transiently expresses GFP-binding CluMPS (LaG17-mCh-HOTag3) in mammalian cells and lentiviral vector for constitutive expression.

   Depositor

   Lukasz Bugaj

   Article

   Mumford et al Cell Syst. 2024 Feb 21;15(2):166-179.e7.

   Insert

   LaG17 CluMPS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRNA-CBh-eGFP

   Plasmid

   #206885

   Purpose

   cloning backbone for sgRNA using OptScf2

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPFO*

   Plasmid

   #120400

   Purpose

   Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

   Depositor

   Arun Radhakrishnan

   Article

   Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

   Insert

   Mutant form of Perfringolysin O
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Amino acids 1-27 are deleted, starts at amino aci…

   Promoter

   Available Since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. IL2Rα-EGFP

   Plasmid

   #86055

   Purpose

   A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   Interleukin-2 receptor subunit alpha (IL2RA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAVTRE3_tdTomato

   Plasmid

   #135178

   Purpose

   AAV expression of TRE(TRE3G)-driven, tdTomato expression for fluorescent labling

   Depositor

   Tetsuo Yamamori

   Article

   Abe et al Front Neuroanat. 2018 Oct 30;12:89. doi:

   Inserts

   tdTomato
   TRE3G
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   a variant of DsRed

   Promoter

   TRE3G

   Available Since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCerulean-VSVG

   Plasmid

   #11913

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

   Insert

   VSVG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   F64L, S65T, Y66W, S72A, N146I, Y145A, H148D, M15…

   Promoter

   Available Since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. iRFP682-Smad2

   Plasmid

   #118943

   Purpose

   Mammalian expression of human Smad2 protein

   Depositor

   Won Do Heo

   Article

   Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

   Insert

   iRFP682-Smad2 (SMAD2 Human)
   

   Use

   Tags

   iRFP682

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLHCX-FLAG-mPKM1

   Plasmid

   #44240

   Depositor

   Lewis Cantley, Matthew Vander Heiden

   Article

   Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

   Insert

   Pyruvate Kinase M1 (Pkm Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

10. Myc-βarrestin2-LOV-Turbo_pCDNA3

    Plasmid

    #199671

    Purpose

    expresses Myc-βarrestin2-LOV-Turbo in mammalian cells

    Depositor

    Alice Ting

    Article

    Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

    Insert

    LOV-Turbo
    

    Use

    Tags

    Myc and βarrestin2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLIK 3xFLAG-CSE1L zeo

    Plasmid

    #192320

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-CSE1L

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

    Insert

    CSE1L (CSE1L Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. ER-NFAST

    Plasmid

    #233596

    Purpose

    Expression of NFAST on the ER membrane

    Depositor

    Riccardo Filadi

    Article

    Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

    Insert

    NFAST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-hSyn-DIO-GRAB_eCB2.0

    Plasmid

    #164606

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in Cre positive neurons

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    S383T

    Promoter

    hSyn

    Available Since

    Feb. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEXtev-HMG54kd

    Plasmid

    #26030

    Depositor

    Johann Deisenhofer

    Article

    Istvan et al EMBO J. 2000 Mar 1. 19(5):819-30.

    Insert

    3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Catalytic domain (amino acids 426-488) Methion…

    Promoter

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. CAGGS-EGFP-NLS-3XFLAG-V5

    Plasmid

    #196090

    Purpose

    Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

    Depositor

    Mauro Calabrese

    Article

    Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

    Insert

    GFP
    

    Use

    Tags

    C-terminal 3xFLAG and C-terminal V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAGGs

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-CHRM3-cpHalo∆-mEGFP

    Plasmid

    #194678

    Purpose

    CMV driven expression of the m3 GPCR recorder in mammalian cell lines fused to mEGFP

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    CHRM3_cpHalo∆
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLR5-CAG-44B12mut23(Ser5ph)-SNAP

    Plasmid

    #174877

    Purpose

    PiggyBac transposon vector for expression of RNAPII Ser5ph mintbody

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    RNAPII Ser5ph mintbody-SNAP
    

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWPI-GFP-3xFLAG

    Plasmid

    #201639

    Purpose

    Over-expression of 3xFLAG-tagged GFP

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX6P-1-eIF4E K119A

    Plasmid

    #199440

    Purpose

    Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

    Depositor

    Shin-ichi Hoshino

    Article

    Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

    Insert

    1977 (EIF4E Human)
    

    Use

    Tags

    GST-fusion protein

    Expression

    Bacterial

    Mutation

    changed lysine 119 to alanine

    Promoter

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS puro human-GFP-RLIM-IRES-mCherry

    Plasmid

    #232243

    Purpose

    Mammalian expression of RLIM-GFP-IRES-mCherry

    Depositor

    Francisco Bustos

    Article

    Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

    Insert

    RLIM-GFP-IRES-mCherry (RLIM Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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