We narrowed to 8,732 results for: Set

1. pAAV-2Aneo v2

   Plasmid

   #80033

   Purpose

   Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

   Depositor

   Hiroyuki Konishi

   Article

   Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

   Type

   Empty backbone

   Use

   AAV and Cre/Lox; A platform vector to construct t…

   Available Since

   Aug. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAGM55273

   Plasmid

   #153211

   Purpose

   Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV/Guide-mCherry

   Plasmid

   #170543

   Purpose

   Expresses mCherry and contains a cassette for U6 driven sgRNA transcription

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

   Insert

   mCherry
   

   Use

   CRISPR and Lentiviral

   Promoter

   Ef1-alpha

   Available Since

   Jan. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pR26 CAG/BFP Dest

   Plasmid

   #74282

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTetO-hOSK-CASH-1

   Plasmid

   #188977

   Purpose

   A CASH-1 GSH targeting, doxycycling inducible humanized Oct4, Sox2, and Klf4 expressing CRISPR/Cas9 donor plasmid.

   Depositor

   Sangdun Choi

   Article

   Javaid et al Stem Cells Int. 2022 Sep 22;2022:4537335

   Insert

   Doxycycline inducible reprogramming donor cassette
   

   Expression

   Mammalian

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEND-446_Cacnes_ProA

   Plasmid

   #225609

   Purpose

   Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJ204-loxP-100RPU.2-ETN-BGHpA-cHS4-100RPU.2-ETN-lox2272

   Plasmid

   #154834

   Purpose

   Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of etanercept

   Depositor

   Lise Marie Grav

   Article

   Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

   Inserts

   ETN
   ETN
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   100RPU.2

   Available Since

   Aug. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-tight-DDdCas9GFP-IRES-Neo

   Plasmid

   #102907

   Purpose

   PiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9GFP-IRES-Neo
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   TRE-tight

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV_SynP_DIO_tAKARα _WPRE

   Plasmid

   #196675

   Purpose

   AAV plasmid for mammalian Cre-dependent expression of tAKARα (genetically encoded sensor for protein kinase A).

   Depositor

   Haining Zhong

   Article

   Ma et al Nature. 2022 Nov;611(7937):762-768. doi:

   Insert

   tAKARα
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDGE651

    Plasmid

    #153229

    Purpose

    Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

    Depositor

    Sylvestre Marillonnet

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLD-puro-2A-rtTA-TcVA

    Plasmid

    #24592

    Depositor

    Jason Moffat

    Article

    Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

    Insert

    Gateway(TM) cassette
    

    Use

    Lentiviral

    Tags

    VA

    Expression

    Mammalian

    Available Since

    Aug. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI SMN1

    Plasmid

    #72286

    Purpose

    SMN1 exon 7 splicing cassette

    Depositor

    Elliot Androphy

    Article

    Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

    Insert

    SMN1 (SMN1 Human)
    

    Expression

    Mammalian

    Mutation

    exons 6-7-8 including introns

    Promoter

    CMV

    Available Since

    Jan. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

13. Zebrabow-GateDest

    Plasmid

    #118221

    Purpose

    For cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.

    Depositor

    Albert Pan, Alex Schier

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L - ccdB survival cassette
    

    Use

    Zebrafish expression

    Available Since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEC3006 (pSpy1C-PZn_ffluc)

    Plasmid

    #218515

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Expression

    Bacterial

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PZn (PczcD)

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKHR8

    Plasmid

    #74625

    Purpose

    a plasmid derived from pKHR5: in addition to Venus reporter gene, it also contains cmlc2::mCherry (red heart) reporter gene at 3’ end of the mcs as pKHR7. Genbank accession number is KU144825.

    Depositor

    David Grunwald

    Article

    Hoshijima et al Dev Cell. 2016 Mar 21;36(6):654-67. doi:

    Inserts

    FRT-alpha-crystallin::Venus-FRT-loxP reporter cassette
    cmlc2::mCherry
    

    Use

    Cre/Lox; Zebrafish expression

    Promoter

    alpha-crystallin and cmlc2

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pv6_CBX1_2xChromo

    Plasmid

    #179393

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of CBX1_2xChromo

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    CBX1_2xChromo
    

    Tags

    AviTag and EGFP

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pNOC-CRISPR-GFP

    Plasmid

    #100009

    Purpose

    Expresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneome

    Depositor

    Eva Farre

    Article

    Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

    Type

    Empty backbone

    Use

    Algae, nannochloropsis expression

    Tags

    Cas9-GFP

    Promoter

    Ribi promoter

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMGS54 (AID-P2A-Hygromycin)

    Plasmid

    #126583

    Purpose

    An AID-P2A-HygR cassette to tag the protein of interests with auxin-inducible degron at the c-terminal

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    AID-P2A-HygR
    

    Use

    Other

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. HSC1-GiP

    Plasmid

    #58254

    Purpose

    This self-inactivated retroviral vector has an internal human EF1alpha promoter driving the expression of a bicistronic EGFP ires Puromycin cassette

    Depositor

    James Ellis

    Article

    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    Human EF1-alpha

    Available Since

    Oct. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNCS tdTomato

    Plasmid

    #91767

    Purpose

    Bacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)

    Depositor

    Erik Rodriguez, Roger Tsien

    Article

    Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

    Insert

    tandem dimer Tomato
    

    Tags

    Hexa-Histidine tag, Xpress epitope for detection …

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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