We narrowed to 87,573 results for: cloning

1. pKO (Apr)

   Plasmid

   #110088

   Purpose

   Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Bacterial knockout generation

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCZGY2850

   Plasmid

   #87758

   Purpose

   Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.

   Depositor

   Andrew Chisholm

   Article

   Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

   Insert

   Pcol-19-PH-miniSOG(Q103L)
   

   Tags

   PH

   Expression

   Worm

   Mutation

   Q103L

   Promoter

   Pcol-19

   Available Since

   March 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-secAnxaV-NS

   Plasmid

   #67718

   Purpose

   Gateway entry clone containing secreted human AnxaV with no stop codon

   Depositor

   Rob Parton

   Article

   Morsch et al Front Cell Neurosci. 2015 Aug 31;9:321.

   Insert

   secAnxaV-NS
   

   Expression

   Mammalian

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO V5 HSPA6

   Plasmid

   #19513

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   heat shock 70kDa protein 6 (HSPA6 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5-FRT-GFP-mCherry-3pGW

   Plasmid

   #53965

   Purpose

   Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

   Depositor

   Saeed Tavazoie

   Article

   Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

   Inserts

   GFP
   mCherry
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1/Myc-His(-)-MSulf-1

   Plasmid

   #13007

   Depositor

   Steven Rosen

   Article

   Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

   Insert

   mouse sulfatase 1 (Sulf1 Mouse)
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pDD317 (Pmyo-2::GFP + SEC)

   Plasmid

   #73344

   Purpose

   SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

   Insert

   Pmyo-2::GFP + SEC
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Promoter

   myo-2

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKO (Hyg)

   Plasmid

   #110087

   Purpose

   Suicide vector for generation of gene deletions in mycobacteria. Contains the two genes sacB and galK for negative selection.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Bacterial knockout generation

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFD115

   Plasmid

   #124768

   Purpose

   pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector

   Depositor

   David Bikard

   Article

   Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

   Inserts

   Ptet
   cas9
   term PpflB sgRNA (BsaI)
   

   Use

   CRISPR

   Promoter

   Ptet

   Available Since

   Aug. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCCl-MCS_miniCMV_miRFP703

    Plasmid

    #134985

    Purpose

    The plasmid includes a miRFP703 gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    miRFP703
    

    Use

    Lentiviral

    Promoter

    minCMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCPP5741

    Plasmid

    #128722

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcV-hopV1
    

    Expression

    Bacterial

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCPP5910

    Plasmid

    #128717

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcO-hopO1-1
    

    Expression

    Bacterial

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCPP5058

    Plasmid

    #128710

    Purpose

    Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

    Depositor

    Alan Collmer

    Article

    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do

    Insert

    shcF-hopF2
    

    Expression

    Bacterial

    Available Since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA type III TGF beta receptor (R3-OFF)

    Plasmid

    #10913

    Depositor

    Bob Weinberg

    Article

    Wang et al Cell. 1991 Nov 15. 67(4):797-805.

    Insert

    R3-OFF (Tgfbr3 Rat)
    

    Expression

    Mammalian

    Available Since

    Nov. 11, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA5/FRT/TO HSPA6

    Plasmid

    #19459

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    HSPA6 (HSPA6 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 7, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXPR_124

    Plasmid

    #133461

    Purpose

    EF1a promoter expresses SpyoCas9 and P2A provides EGFP

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    SpyoCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCCl-MCS_miniCMV_TagBFP

    Plasmid

    #134986

    Purpose

    The plasmid includes a TagBFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    TagBFP
    

    Use

    Lentiviral

    Promoter

    minCMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRL1383a

    Plasmid

    #70692

    Purpose

    RSF1010-based vector with extensive polylinker, and resistance to Strep and Spec. Shuttle vector suitable for transfer to, and replication in, E. coli and Anabaena (Nostoc) sp. strain PCC 7120.

    Depositor

    Peter Wolk

    Article

    Wolk et al Arch Microbiol. 2007 Dec;188(6):551-63.

    Type

    Empty backbone

    Use

    Bacterial shuttle vector

    Available Since

    Jan. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDIS3

    Plasmid

    #92173

    Purpose

    Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.

    Depositor

    Judith Berman

    Article

    Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

    Insert

    NAT1
    

    Use

    Genbank: u03440.1

    Expression

    Yeast

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWBC

    Plasmid

    #15239

    Depositor

    Ron Vale

    Article

    Niclas et al Neuron. 1994 May . 12(5):1059-72.

    Insert

    Human neuronal kinesin heavy chain (KIF5A Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 12, 2007

    Availability

    Academic Institutions and Nonprofits only