We narrowed to 9,470 results for: pho;

1. pUG36-NES-EGFP-cPHx3

   Plasmid

   #183668

   Purpose

   PtdIns(3,4)P2 biosensor for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLEKHA1 (PLEKHA1 Frog, Human)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Yeast

   Mutation

   Amino acids 169-329 (tandem trimer)

   Promoter

   MET25

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUG34-NES-EGFP-cPHx3

   Plasmid

   #183669

   Purpose

   PtdIns(3,4)P2 biosensor for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLEKHA1 (PLEKHA1 Frog, Human)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Yeast

   Mutation

   Amino acids 169-329 (tandem trimer)

   Promoter

   MET25

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NES-mCherry-PH-BTKx1

   Plasmid

   #183653

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Frog, Human)
   

   Use

   Tags

   X. laevis map2k1.L(32-44)-mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-170

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-N-TEV-Twin-Strep

   Plasmid

   #222014

   Purpose

   Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in E.coli

   Depositor

   Chiaki Murakami

   Type

   Empty backbone

   Use

   Tags

   TEV cleavable site (ENLYFQG) and Twin-strep-tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac promoter

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAcGFP-C1-DGKd2

   Plasmid

   #224897

   Purpose

   Express N-terminal mCherry fused to human diacylglycerol kinase delta isoform 2 in mammalian cells

   Depositor

   Chiaki Murakami

   Insert

   DGKD (DGKD Human)
   

   Use

   Tags

   AcGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-Sfp R4-4 mutant

   Plasmid

   #173491

   Purpose

   Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

   Depositor

   Jun Yin

   Article

   Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

   Insert

   Sfp-R44
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p15aC-1D-5

   Plasmid

   #107865

   Purpose

   Bacterial expression of PIS and PI4P5K

   Depositor

   Sanford Simon

   Article

   Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

   Inserts

   PIS
   phosphatidylinositol 4-phosphate 5-kinase
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   Promoter

   proD and proD (in operon)

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HGXPRT

   Plasmid

   #64201

   Purpose

   Expression of Plasmodium falciparum HGXPRT

   Depositor

   Vern Schramm

   Article

   Hazleton et al Chem Biol. 2012 Jun 22;19(6):721-30. doi

   Insert

   Hypoxanthine guanine xanthine phosphoribosyltransferase
   

   Use

   Tags

   Histidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pRK5-PLBD2-6xHis

   Plasmid

   #213606

   Purpose

   Expresses PLBD2-6xHis in mammalian cells

   Depositor

   Monther Abu-Remaileh

   Article

   Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

   Insert

   phospholipase B domain containing 2 (PLBD2 Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAGGS-Twin-strep-TEV-DGKδ2

    Plasmid

    #223720

    Purpose

    Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

    Depositor

    Chiaki Murakami

    Insert

    DGKD (DGKD Human)
    

    Use

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted start codon

    Promoter

    CAG and chicken β-actin promoter

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge