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Plasmid#118528PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmOrange2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PIK3CA - Exon20 H1047R (pFastBac)
Plasmid#16641DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseBaculoviralTagsHisExpressionMammalianMutationH1047RPromoterAvailable sinceMarch 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + PrgJ
Plasmid#84869PurposeBaculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
PrgJ
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)
Plasmid#110561PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insertDepositorInsertstRBS-aacC1
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(T308A/S473A)
Plasmid#86578PurposeExpresses human full-length Akt1(T308A/S473A) phosphomutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationT308A/S473A phosphomutantPromoterAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT S6K (E389-deltaCT)
Plasmid#58507PurposeGateway entry vector for an inducible HA-tagged rat S6K1-E389-deltaCTDepositorInsertRps6kb1 (Rps6kb1 Rat)
UseEntry vectorTagsHAExpressionMutationT389E, stop codon at amino acid 402PromoterAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB222
Plasmid#68613PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB219
Plasmid#68610PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-GST
Plasmid#39287DepositorInsertnmt1 promoter (nmt1 Fission Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…ExpressionMutation-1163 to +6 of nmt1 locus with a 4bp deletion (TA…PromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(K14A)
Plasmid#86562PurposeExpresses human full-length Akt1(K14A) mutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationK14A mutation in the PH domainPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB221
Plasmid#68612PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)
Plasmid#79031PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(African green monkey pygerythrus)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(D323A/D325A)
Plasmid#86592PurposeExpresses human full-length Akt1(D323A/D325A) mutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationD323A/D325A transforming mutationsPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pfast bac-flag Fbxl3
Plasmid#51894PurposeBackbone plasmid is pfastBacHTa from invitrogenDepositorInsertFbxl3 (Fbxl21 Mouse)
UseTagsFLAGExpressionMutationPromoterpolyhedrin promoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmRE-Tn5-142
Plasmid#118531PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3xFLAG SMAD4
Plasmid#83094PurposeGateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tagDepositorInsertSMAD4 (SMAD4 Human)
UseEntry vector for gateway cloningTags3xFLAGExpressionMutationPromoterTRE tight promoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEBAC1-CoREST-C
Plasmid#109159PurposeEncodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)
UseTags3xFLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-LacI-LAP-HEC1
Plasmid#114033PurposeProduction of baculovirus with LacI-LAP-HEC1DepositorInsertLacI-LAP-HEC1 (NDC80 Human)
UseTagsExpressionInsectMutationnonePromoterCMVAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_N_Strep
Plasmid#101651PurposeExpression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, KpnI linker, Twin-Strep tag
UseTagsTwin-Strep tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_C_Strep
Plasmid#101652PurposeExpression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cellsDepositorInsertLRP6 Signal peptide, MCS linker, Twin-Strep tag
UseTagsTwin-Strep tagExpressionInsectMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only