Addgene

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pBS-SKII-3XHA-eDHFR-Hygromycin

Plasmid

#188924

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-eDHFR-Hygromycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Phleomycin

Plasmid

#188920

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Phleomycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-Hygromycin

Plasmid

#188919

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Hygromycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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JME5000

Plasmid

#129661

Purpose

EYK1ex_CrisprCas9-yl_RFP: CAS9 vector with EYK1ex marker for gRNA cloning using GoldenGate

Depositor

Tristan Rossignol

Article

Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCMB-TGNVHAr

Plasmid

#61179

Purpose

Fluorescent reporter for trans-Golgi network AtVHA-a1-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtVHA-a1

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-GAr

Plasmid

#61173

Purpose

Fluorescent reporter for Golgi apparatus GmMAN49-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

GmMAN49-mCherry

Use

Tags

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

April 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-MITr

Plasmid

#61199

Purpose

Fluorescent reporter for motochondria ScCOX4sp-mCherry expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

ScCOX4sp-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-TGN41r

Plasmid

#61177

Purpose

Fluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtSYP41

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-LEr

Plasmid

#61186

Purpose

Fluorescent reporter for late endosome mCherry-MtRAB5A2 expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtRAB5A2

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCMB-ERr

Plasmid

#61171

Purpose

Fluorescent reporter for endoplasmic reticulum AtWAK2sp-mCherry-HDEL expressed under MtBCP1 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtWAK2-mCherry-HDEL

Use

Tags

Expression

Plant

Mutation

Promoter

MtBCP1

Available since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-mTurquoise2

Plasmid

#98886

Purpose

Produces a fusion between mTurquoise2 and mNeonGreen. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pPROEX Aqua

Plasmid

#42889

Depositor

Fabienne Merola

Article

Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

Insert

Aquamarine

Use

Tags

6xHis and TEV cleavage

Expression

Bacterial

Mutation

T65S and H148G

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pSBbi 41BBL-CD86

Plasmid

#183244

Purpose

Expression of human 41BBL and CD86 in a Sleeping Beauty transposon vector for use in artificial antigen presenting cells

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

CD86, 41BBL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-HALO-3xHA

Plasmid

#188937

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-HALO-3xHA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSLC-237

Plasmid

#73191

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

ParaB-relE

Use

Tags

Expression

Bacterial

Mutation

Promoter

ParaB

Available since

June 30, 2016

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-2A-mTurquoise2

Plasmid

#98885

Purpose

Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mNeonGreen-2A-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pUCBB-oriT

Plasmid

#32556

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

oriT

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pTE102

Plasmid

#59397

Purpose

MethyloBrick expression vector, mxaF promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PmxaF

Available since

Dec. 30, 2014

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-mTurquoise2

Plasmid

#98839

Purpose

Produces a fusion between mTurquoise2 and mScarletI. It can be used as a positive control for FRET

Depositor

Dorus Gadella

Article

Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

Insert

mScarletI-mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSLC-246

Plasmid

#73190

Purpose

Template plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (tse2) under the control of rhamnose induceable promoter (PrhaB) for negative selection.

Depositor

Swaine Chen

Article

Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8

Insert

PrhaB-tse2

Use

Tags

Expression

Bacterial

Mutation

Promoter

PrhaB

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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