We narrowed to 2,288 results for: Tub;

1. pYC2085

   Plasmid

   #158721

   Purpose

   CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   Sth1 sgRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNHEJ-recAmu-sacB

   Plasmid

   #158717

   Purpose

   CRISPR-assisted-NHEJ system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses NHEJ machinery of M. marinum and recAR61C allele.

   Depositor

   Yi-Cheng Sun

   Article

   Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

   Insert

   recA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   R61C

   Promoter

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. MAC_N_TBA1A

   Plasmid

   #111695

   Purpose

   MAC-tagged gene expression

   Depositor

   Markku Varjosalo

   Article

   Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

   Insert

   TBA1A (TUBA1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Plasmid L5 attB:Psmyc double green

   Plasmid

   #169412

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for double green

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   mWasabi
   mWasabi CO
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Plasmid L5 attB:Psmyc triple red

   Plasmid

   #169413

   Purpose

   Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple red

   Depositor

   Clifton Barry III

   Article

   Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

   Inserts

   mScarlet proper
   COmScarlet
   mRuby3
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SRS-MTBD(22/19), AB(monomer), wt

   Plasmid

   #22380

   Depositor

   Ron Vale

   Article

   Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

   Insert

   Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
   

   Use

   Tags

   His and myc

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET21a - Mtb-TF

   Plasmid

   #111513

   Purpose

   Expresses M. tuberculosis Trigger Factor with a C-terminal His tag

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

   Insert

   Trigger Factor
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SRS-MTBD(26/19) AB(monomer), wt

   Plasmid

   #22381

   Depositor

   Ron Vale

   Article

   Gibbons et al J Biol Chem. 2005 Jun 24. 280(25):23960-

   Insert

   Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
   

   Use

   Tags

   myc

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SRS-MTBD(26/19)ux, dimer, no myc

   Plasmid

   #22382

   Depositor

   Ron Vale

   Article

   Carter et al Science. 2008 Dec 12. 322(5908):1691-5.

   Insert

   Seryl tRNA synthetase (SRS) – mouse cytoplasmic dynein microtubule binding domain (MTBD) fusion protein
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 24, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. His-FGE

    Plasmid

    #75147

    Purpose

    Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.

    Depositor

    Richard Fishel

    Article

    Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10

    Insert

    FGE
    

    Use

    Tags

    Histidine tag and Thrombin

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    T7

    Available since

    July 8, 2016

    Availability

    Academic Institutions and Nonprofits only

11. SspBmicro-mCh-p60

    Plasmid

    #190168

    Purpose

    Expresses SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    SspBmicro-mCherry-p60
    

    Use

    Tags

    SspBmicro-mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-αTAT1[2-236]

    Plasmid

    #27101

    Depositor

    Maxence Nachury

    Article

    Shida et al Proc Natl Acad Sci U S A. 2010 Nov 10. (

    Insert

    αTubulin K40 acetyltransferase (ATAT1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. TagRFP-T_A1aY1

    Plasmid

    #158751

    Purpose

    Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    TagRFP-T_A1aY1
    

    Use

    Lentiviral

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (CMV enhancer and chicken beta actin promoter)

    Available since

    Oct. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSIN-TRE-SspB-mCh-p60

    Plasmid

    #190170

    Purpose

    Doxycycline-inducible lentivirus vector to express SspBmicro-mCh-p60, a recruitable katanin for microtubule severing, in mammalian cells.

    Depositor

    Anna Akhmanova

    Article

    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

    Insert

    SspBmicro-mCherry-p60
    

    Use

    Lentiviral

    Tags

    SspBmicro-mCherry

    Expression

    Mutation

    Promoter

    Available since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. plRL58

    Plasmid

    #166886

    Purpose

    Optimized M. tuberculosis CRISPRi plasmid; contains an L5 attP region (no L5 Integrase) that allows for integration when co-transformed with an L5 Integrase delivery plasmid (e.g. plRL19, ID #163634)

    Depositor

    Jeremy Rock

    Article

    Bosch et al Cell. 2021 Jul 16. pii: S0092-8674(21)00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet-On

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TagBFP_A1aY1

    Plasmid

    #158752

    Purpose

    Lentiviral vector for expression of TagBFP_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

    Depositor

    Minhajuddin Sirajuddin

    Article

    Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

    Insert

    TagBFP-T_A1aY1
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG (CMV enhancer and chicken beta actin promoter)

    Available since

    Sept. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHis-hMTMR1

    Plasmid

    #100723

    Purpose

    Expression of human MTMR1 PH-PTP domain

    Depositor

    Byung Il Lee

    Article

    Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

    Insert

    MTMR1 (MTMR1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 95-607

    Promoter

    T7

    Available since

    Nov. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHAGE-UBC-TagRFP-T-ps

    Plasmid

    #178656

    Purpose

    Expression of palmitoylated TagRFP-T for live imaging of plasma membrane (protrusions, tunneing nanotubes), in mammalian cells

    Depositor

    Jeffrey Gerst

    Article

    Haimovich et al Proc Natl Acad Sci U S A. 2017 Nov 14;11

    Insert

    TagRFP-T with palmitoylation signal at N-terminus
    

    Use

    Lentiviral

    Tags

    Palmitoylation signal

    Expression

    Mutation

    Promoter

    Ubc

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GST-TPX2(1-43)

    Plasmid

    #69107

    Purpose

    For bacterial expression of human TPX2(1-43aa) tagged with GST.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    Targeting Protein for Xklp2 (TPX2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Amino acids 1-43 expressed.

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCZGY1584 Pcol-19::ebp-2::mGFP::unc-54 3'UTR

    Plasmid

    #129774

    Purpose

    Marker of microtubule dynamics in C. elegans hypodermis

    Depositor

    Andrew Chisholm

    Article

    Chuang et al Elife. 2016 Sep 23;5. doi: 10.7554/eLife

    Inserts

    Pcol-19
    ebp-2 (ebp-2 Nematode)
    unc-54 3'UTR
    

    Use

    Synthetic Biology; Gateway

    Tags

    Synthetic Intron and mGFP

    Expression

    Worm

    Mutation

    Promoter

    Pcol-19

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge