We narrowed to 9,698 results for: ef1

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pHL-EF1a-SphcCas9-iP-A

Plasmid

#60599

Purpose

Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

Depositor

Akitsu Hotta

Article

Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

Insert

CRISPR Cas9

Use

CRISPR

Expression

Mammalian

Mutation

Codon usage optimized for human usage

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112924

Purpose

piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Piggybac transposon vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pVH21-Tier1-PhEF1a

Plasmid

#169561

Purpose

Tier-1 PhEF1a-driven expression vector (PhEF1a-MCS-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1aL-dsRed-W

Plasmid

#195863

Purpose

lentiviral expression of the red fluorescent protein driven by human EF1aL promoter

Depositor

Darrell Kotton

Type

Empty backbone

Use

Lentiviral

Tags

dsRed

Promoter

EF1aL

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR221-EF1.Pro

Plasmid

#79488

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human EF1 promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

EF1 Promoter (EEF1A1 Human, Synthetic)

Use

Multisite gateway entry clone

Promoter

EF1 Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEF1a-Usp9x_CD-mCherry

Plasmid

#170380

Purpose

Vector for mammalian expression of the Usp9x catalytic domain tagged with mCherry, in pEF1a-IRES-Neo backbone.

Depositor

Miguel Ramalho-Santos

Article

Macrae et al Nat Commun. 2021 Mar 25;12(1):1865. doi:

Insert

Ubiquitin specific protease 9x catalytic domain (Usp9x Mouse)

Tags

mCherry-3xFlag

Expression

Mammalian

Mutation

Catalytic domain

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 EF1a-EGFP

Plasmid

#231925

Purpose

3rd generation lentiviral transfer plasmid with EGFP reporter gene

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

EGFP

Use

Lentiviral

Promoter

EF-1a

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Bet1

Plasmid

#120243

Purpose

Expresses V5-tagged Bet1 in lentiviral vector

Depositor

Larry Gerace

Insert

Bet1 (Bet1 Mouse)

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pAdx-EF1-tdTomato

Plasmid

#73354

Purpose

Express tdTomato under EF1 promoter

Depositor

Kazuhiro Oka

Insert

EF1-tdTomato

Use

Adenoviral

Promoter

EF-1

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pSH231-EF1-Puro

Plasmid

#115143

Purpose

Safe harbor site 231 knock-in vector with puromycin resistance selection cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Puromycin

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-sCre

Plasmid

#55635

Purpose

Expresses sCre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

Plasmid

#174899

Purpose

Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pT3-EF1A-CCNE2

Plasmid

#162554

Purpose

Overexpression of CCNE2

Depositor

Amaia Lujambio

Article

Molina-Sanchez et al Gastroenterology. 2020 Aug 16. pii: S001

Insert

Overexpression of CCNE2 (CCNE2 Human)

Expression

Mammalian

Mutation

None

Promoter

EF1A

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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mc2 mNeon EF1a

Plasmid

#206224

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mLef1 (CT#1141)

Plasmid

#14021

Depositor

Cliff Tabin

Insert

Lef1 (Lef1 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pCDH-EF1-Nluc

Plasmid

#73024

Purpose

Express Nluc reporter under EF1 promoter

Depositor

Kazuhiro Oka

Insert

Nluc

Use

Lentiviral

Expression

Mammalian

Promoter

EF1

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-N-cG-Neo

Plasmid

#124978

Purpose

Express Nanoluc, copGFP

Depositor

Kazuhiro Oka

Insert

Nanoluc, copGFP

Use

Lentiviral

Promoter

EF1-alpha

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->sGFP_PGK-->puroR

Plasmid

#162156

Purpose

Lentiviral vector for constitutive expression of GFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP

Use

Lentiviral

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α1.1-tdTomato

Plasmid

#122101

Purpose

AAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

tdTomato

Use

AAV

Tags

NA

Expression

Mammalian

Promoter

EF1a（1.1kb short version)

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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