We narrowed to 12,024 results for: pcdna

1. PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry

   Plasmid

   #124493

   Purpose

   Expresses FlipYFP (TEV cleavage sequence) and T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipYFP(TEV cleavage seq) T2A mCherry
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1/Puro-CAG-JEDI-1P-P2A-mCherry-CAAX

   Plasmid

   #202606

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P fused to membrane-anchored mCherry via P2A, expressed under strong mammalian promoter (CAG)

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P-P2A-mCherry-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. (344) pcDNA3.1-HA-nGFP-(EAAAK)4-OGA(544-706)

   Plasmid

   #168097

   Purpose

   HA- tagged GFP nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA.

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nGFP-(EAAAK)4-OGA(544-706)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

   Plasmid

   #188994

   Purpose

   A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN

   Depositor

   Jeremy Baskin

   Article

   Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

   Insert

   PLD
   

   Use

   Tags

   CAAX, CIBN, CRY2, P2A, and mCherry

   Expression

   Mammalian

   Mutation

   K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…

   Promoter

   CMV

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG

   Plasmid

   #199328

   Purpose

   mutated RHIM motive (interaction inhibited)

   Depositor

   Adrian Press

   Article

   Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

   Insert

   Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   changed V458, Q459,V460 and G461 to alanine

   Promoter

   Available Since

   June 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. (271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

   Plasmid

   #168102

   Purpose

   HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

   Depositor

   Christina Woo

   Article

   Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

   Insert

   HA-nEPEA-(EAAAK)3-OGA(544-706)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   OGA truncated to contain residues 544-706

   Promoter

   T7/CMV

   Available Since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA type III TGF beta receptor (R3-OFF)

   Plasmid

   #10913

   Depositor

   Bob Weinberg

   Article

   Wang et al Cell. 1991 Nov 15. 67(4):797-805.

   Insert

   R3-OFF (Tgfbr3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

8. pOTTC1755 - pcDNA3.1 CMV-IE hChR2(H134R)-V5-ER-LBD (ChRERa)

   Plasmid

   #201819

   Purpose

   A plasmid expressing channelrhodopsin-2 fused to a V5 epitope and the ligand-binding domain of estrogen receptor (ChRERa) from a CMV promoter

   Depositor

   Christopher Richie

   Article

   Bonaventura et al Sci Transl Med. 2023 Jul 26;15(706):eadd

   Insert

   hChR2(H134R)-V5-ER-LBD (ChRERa)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pOTTC1751

   Available Since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+) CircRNA Mini Vector-ZKSCAN1 nt 548-1047

   Plasmid

   #60649

   Purpose

   Expresses a miniature version of the ZKSCAN1 circular RNA

   Depositor

   Jeremy Wilusz

   Article

   Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX

    Plasmid

    #188991

    Purpose

    A plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBN

    Depositor

    Jeremy Baskin

    Article

    Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

    Insert

    PLD
    

    Use

    Tags

    CAAX, CIBN, CRY2, P2A, and mCherry

    Expression

    Mammalian

    Mutation

    F163L, K249R, G328S, G381V, G429D, D480G

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Antisense IRES

    Plasmid

    #69910

    Purpose

    Expresses a GFP circular RNA containing the antisense EMCV IRES in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    ZKSCAN1, GFP (ZKSCAN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5 FRT/TO/STRP 3x FLAG cyclin B1 L45A (1391)

    Plasmid

    #61841

    Purpose

    mammalian expression of cyclin B1 non-degradable L45A mutant

    Depositor

    Jonathon Pines

    Article

    Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

    Insert

    cyclin B1 (CCNB1 Human)
    

    Use

    Tags

    2xSTREP and 3xFLAG

    Expression

    Mammalian

    Mutation

    L45A

    Promoter

    CMV

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut

    Plasmid

    #42046

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    FLAG, HA, and SBP

    Expression

    Mammalian

    Mutation

    F951A, F955A, I958A, F960A

    Promoter

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. (424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)

    Plasmid

    #168191

    Purpose

    HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutation

    Depositor

    Christina Woo

    Article

    Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

    Insert

    HA-nLag6-(EAAAK)4-OGT(4,K852A)
    

    Use

    Tags

    HA-Tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7/CMV

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3_attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA_DEST

    Plasmid

    #194386

    Purpose

    Gateway Destination vector for GPCR split TEV assays with V2R tail

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    attR1-ORF-attR2-V2R-NTEV-TCS-GV-2xHA
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA-gp78-FLAG RING mutant C356G H361A

    Plasmid

    #61751

    Purpose

    express C-terminally FLAG tagged human gp78

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    gp78 (AMFR Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C356G H361A; P179L, F262L, Y315C, and D346G (ple…

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NLS-mut

    Plasmid

    #42045

    Depositor

    Kumiko Ui-Tei

    Article

    Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

    Insert

    TNRC6A (TNRC6A Human)
    

    Use

    Tags

    FLAG, HA, and SBP

    Expression

    Mammalian

    Mutation

    R930A, R931A, R932A, R934A

    Promoter

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-LplA(W37I) Alternative coumarin PRIME ligase

    Plasmid

    #25846

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA-W37I
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    W37I

    Promoter

    Available Since

    July 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL

    Plasmid

    #73209

    Purpose

    Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    kappa-myc-dL5-2XG4S-mCer3-KDEL (MYC Human, Synthetic)
    

    Use

    Tags

    The FAP and mCerulean3 are fused with 2 copies of…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Flag::UbvG08 P69L, L70V, I44A, deltaGG

    Plasmid

    #74940

    Purpose

    Ubiquitin variant with mutations L69P and V70L reverted to wild type (i53-DM mutant)

    Depositor

    Daniel Durocher

    Article

    Canny et al Nat Biotechnol. 2018 Jan;36(1):95-102. d

    Insert

    Ubiquitin
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    UbvG08 with I44A, P69L, and L70V mutations and no…

    Promoter

    CMV

    Available Since

    May 13, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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