We narrowed to 8,732 results for: Set

1. pPBT-PE2-PGK-Blast

   Plasmid

   #173219

   Purpose

   piggyBac transposon containing PE2 and Blasticidin expression cassettes

   Depositor

   Jacob Giehm Mikkelsen

   Article

   Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. d

   Inserts

   PE2
   Blast
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   CMV and hPGK

   Available Since

   May 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLib6.4B-Consensus

   Plasmid

   #176678

   Purpose

   Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Expression

   Insect

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGM65905

   Plasmid

   #153217

   Purpose

   Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAT9658-sgRNA-mCherry

   Plasmid

   #162987

   Purpose

   sgRNA cloning plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTmpKmH207in

   Plasmid

   #176230

   Purpose

   A template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed toward the cI gene.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTmpKmH207out

   Plasmid

   #176231

   Purpose

   A template plasmid for amplification of the cI-hok-neo cassette, which contains a truncated H207 promoter of T5 phage. The promoter is directed outward the cI gene.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, neo, truncated PH207 promoter of T5 phage
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTmpKmA1O4in

   Plasmid

   #176232

   Purpose

   A template plasmid for amplification of the cI-hok-neo cassette, which contains A1O4 promoter. The promoter is directed toward the cI gene.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTmpKmA1O4out

   Plasmid

   #176233

   Purpose

   A template plasmid for amplification of the cI-hok-neo cassette, which contains LacI-repressible A1O4 promoter. The promoter is directed outward the cI gene.

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, neo, hybrid PA1O4 promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYFP-NEAT1pr_v2-RT

   Plasmid

   #97088

   Purpose

   Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v2 vector, but also works with px330-NEAT1pr_v1.

   Depositor

   Archa Fox

   Article

   Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

   Insert

   NEAT1pr_v2-RT (NEAT1 Human)
   

   Use

   CRISPR

   Tags

   EYFP

   Promoter

   CMV

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pv6_EGFP

    Plasmid

    #179392

    Purpose

    Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of EGFP

    Depositor

    Tuncay Baubec

    Article

    Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

    Insert

    EGFP
    

    Tags

    AviTag

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-AAVS1-Ef1a-Zeo

    Plasmid

    #227268

    Purpose

    Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen

    Plasmid

    #200401

    Purpose

    Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    Open-loop DART VADAR sensor entry cassette
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQmod2E-GG

    Plasmid

    #191345

    Purpose

    Clostridium expression vector (pBP1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWPI/hPLK2K111M/Neo

    Plasmid

    #35386

    Depositor

    Anurag Tandon

    Article

    Visanji et al J Biol Chem. 2011 Oct 14;286(41):35863-7

    Insert

    hPLK2 K111M (PLK2 Human)
    

    Use

    Lentiviral

    Tags

    EMCV IRES-Neomycin cassette and V5 epitope and Po…

    Expression

    Mammalian

    Mutation

    K111M

    Promoter

    EF1-alpha

    Available Since

    April 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. attB53-Pac-TRE-mCherry-attB53

    Plasmid

    #183611

    Purpose

    RMCE vector to shuttle puromycin resistance and TRE-mCherry cassettes into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

    Depositor

    Sally Lowell

    Article

    Malaguti et al Development. 2022 May 26. pii: 275525. d

    Insert

    TRE-mCherry
    

    Use

    Rmce

    Promoter

    TRE

    Available Since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAGM55297

    Plasmid

    #153213

    Purpose

    Level 2 cloning vector for a single guide RNA, already contains Cas9, Kanamycin selection cassette for plant transformation and FAST marker

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PGK+ episome

    Plasmid

    #170041

    Purpose

    PGK+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK-tetO2

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. N-terminal Halo-XLF

    Plasmid

    #207546

    Purpose

    Homologous recombination donor to insert halo tag at the N terminal of the endogenous XLF locus.

    Depositor

    Jens Schmidt

    Article

    Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

    Insert

    HaloTag with internal PuroR cassette flanked by human Lig4 locus sequences
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIW447-CRISPR XYL2

    Plasmid

    #98906

    Purpose

    K. marxianus CRISPR Plasmid for XYL2 editing

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV+ episome

    Plasmid

    #169742

    Purpose

    CMV+ circuit on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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